Exbio — Research products — Antibodies — Nucleus — Anti-BrdU Purified

Anti-BrdU Purified

Regulatory status

RUO

Antigen

BrdU

Clone

MoBu-1

Format

Purified

Application

FC (QC tested), IHC-P, ICC

Variant

0.1 mg

11-286-C100

In stock

169.40 USD

0.025 mg

11-286-C025

Delivery 1 week

84.70 USD

Variant

0.1 mg

11-286-C100

In stock

169.40 USD

0.025 mg

11-286-C025

Delivery 1 week

84.70 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It reacts also specifically with 5-bromouridine (BrU), and it does not cross-react with EdU.

Application

FC (QC tested), IHC-P, ICC

Application details

Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining.
Immunocytochemistry: Recommended dilution: 2 μg/ml.
Immunohistochemistry (paraffin sections): Excellent.

Immunogen

5-bromodeoxyuridine conjugated with hemocyanine.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

5-bromodeoxyuridine

Antigen description

Bromodexyuridine (BrdU) is a thymidine analog, which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.

Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM human acute lymphoblastic leukemia cell line using purified anti-5-bromodeoxyuridin (MoBu-1) (detection by Goat anti-mouse IgG1 FITC). The individual cell cycle phases (S-, G1-, G2/M-phase) are indicated in the figure.

Immunohistochemistry staining of bromodeoxyuridine-labeled cells (chick embryo; paraffin-embedded sections) with anti-5-bromodeoxyuridine (MoBu-1).

General references:

Ashby J, Tinwell H, Soames A, Foster J: Induction of hyperplasia and increased DNA content in the uterus of immature rats exposed to coumestrol. Environ Health Perspect. 1999 Oct;107(10):819-22.
PubMed

Soames AR, Lavender D, Foster JR, Williams SM, Wheeldon EB: Image analysis of bromodeoxyuridine (BrdU) staining for measurement of S-phase in rat and mouse liver. J Histochem Cytochem. 1994 Jul;42(7):939-44.
PubMed

Product specific references:

Stanek D, Kiss T, Raska I: Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription. Eur J Cell Biol. 2000 Mar;79(3):202-7.
PubMed

Buckiova D, Kubinova L, Soukup A, Jelinek R, Brown NA: Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects. Int J Dev Biol. 1998 Jul;42(5):737-40.
PubMed

Liboska R, Ligasová A, Strunin D, Rosenberg I, Koberna K: Most anti-BrdU antibodies react with 2'-deoxy-5-ethynyluridine - the method for the effective suppression of this cross-reactivity. PLoS One 2012;7(12):e51679.
PubMed