Exbio — Research products — Antibodies — Nucleus — Anti-beta-Catenin Purified

Anti-beta-Catenin Purified

Anti-beta-Catenin Purified
Regulatory status
RUO
Antigen
beta-Catenin
Clone
EM-22
Format
Purified
Reactivity
Human, Mouse, Hamster
Application
FC, IP, WB, ICC
Variant
0.1 mg
11-557-C100
In stock
180.00 USD
0.025 mg
11-557-C025
Out of stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
0.1 mg
11-261-C100
In stock
90.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody EM-22 reacts with C-terminal part of beta-catenin (intracellular antigen), an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.
Application
FC, IP, WB, ICC
Application details
Immunocytochemistry: Positive control: HT29 human colon adenocarcinoma cell line.
Western blotting: Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line, KW1 murine cell line, C57MG murine cell line, 3T3 murine fibroblast cell line.
Flow cytometry: Intracellular staining; recommended dilution: 1-4 µg/ml.
Reactivity
Human, Mouse, Hamster
Immunogen
Recombinant human beta-catenin
Other names
CTNNB1, EVR7, MRD19
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-557_ICC
Immunocytochemistry staining of beta-catenin in human colon adenocarcinoma cell line HT29 using EM-22 antibody (green). Cell nuclei visualized by DAPI (blue).
11-557 WB
Western blotting analysis of beta-catenin in murine 3T3 (A), C57 (B) and KW1 (C) cell lines using EM-22 antibody.

General references:

Nowak M, Madej JA, Dziegiel P: Expression of E-cadherin, beta-catenin and Ki-67 antigen and their reciprocal relationships in mammary adenocarcinomas in bitches. Folia Histochem Cytobiol. 2007;45(3):233-8.
PubMed
Jung IM, Chung JK, Kim YA, Kim JE, Heo SC, Ahn YJ, Hwang KT, Kim BG, Lee KL, Kim CW, Kim WH, Chang MS: Epstein-Barr Virus, Beta-Catenin, and E-cadherin in Gastric Carcinomas. J Korean Med Sci. 2007 Oct;22(5):855-61.
PubMed
Valenta T, Lukas J, Korinek V: HMG box transcription factor TCF-4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells. Nucleic Acids Res. 2003 May 1;31(9):2369-80.
PubMed
Variant
0.1 mg
11-557-C100
In stock
180.00 USD
0.025 mg
11-557-C025
Out of stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
0.1 mg
11-261-C100
In stock
90.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product