Exbio — Research products — Antibodies — Nucleus — Anti-BCL6 Purified

Anti-BCL6 Purified

Regulatory status

RUO

Antigen

BCL6

Clone

IG191E/A8

Format

Purified

Reactivity

Mouse, Human

Application

IP, WB, FC (QC tested), IHC(P)

Variant

0.1 mg

11-867-C100

In stock

198.00 USD

Variant

0.1 mg

11-867-C100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody IG191E/A8 recognizes BCL6, a transcriptional repressor serving as a master regulator of germinal center reaction.

Application

IP, WB, FC (QC tested), IHC(P)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

Reactivity

Mouse, Human

Immunogen

pCDNA3 plasmid containing full-length sequence of human BCL6

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

LAZ3, ZNF51, ZBTB27

Antigen description

BCL6 is a zinc finger-containing transcription factor, which acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. On B cells, BCL6 induces proliferation, antibody class switching and affinity maturation, whereas it inhibits their differentiation into plasma cells. On T cells, BCL6 induces their differentiation into T-FH cells.

Entrez Gene ID 604

UniProt ID P41182

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human BCL6 (IG191E/A8) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Separation of monocytes stained anti-human BCL6 (IG191E/A8) purified antibody (concentration in sample 5 μg/ml, GAM APC, red-filled) from monocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining)

Product specific references:

Jain S, Stock A, Macian F, Putterman C: A Distinct T follicular helper cell subset infiltrates the brain in murine neuropsychiatric lupus. Front Immunol. 2018 Mar 13;9:487.
PubMed

Hoser D, Schön C, Loddenkemper C, Lohneis P, Kühl AA, Sommermann T, Blankenstein T, Willimsky G: Oncogene-specific T cells fail to eradicate lymphoma-initiating B cells in mice. Blood. 2018 Aug 30;132(9):924-934.
PubMed

Tan HX, Esterbauer R, Vanderven HA, Juno JA, Kent SJ, Wheatley AK: Inducible Bronchus-Associated Lymphoid Tissues (iBALT) Serve as Sites of B Cell Selection and Maturation Following Influenza Infection in Mice. Front Immunol. 2019 Mar 29;10:611.
PubMed

Jiang W, Wragg KM, Tan HX, Kelly HG, Wheatley AK, Kent SJ, Juno JA: Identification of murine antigen-specific T follicular helper cells using an activation-induced marker assay. J Immunol Methods. 2019 Apr;467:48-57.
PubMed