Anti-BCL6 APC

Anti-BCL6 APC
Regulatory status
RUO
Antigen
BCL6
Clone
IG191E/A8
Format
APC
Reactivity
Mouse, Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-867-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody IG191E/A8 recognizes BCL6, a transcriptional repressor serving as a master regulator of germinal center reaction.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
Reactivity
Mouse, Human
Immunogen
pCDNA3 plasmid containing full-length sequence of human BCL6
Preparation
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
LAZ3, ZNF51, ZBTB27
Antigen description
BCL6 is a zinc finger-containing transcription factor, which acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. On B cells, BCL6 induces proliferation, antibody class switching and affinity maturation, whereas it inhibits their differentiation into plasma cells. On T cells, BCL6 induces their differentiation into T-FH cells.
Entrez Gene ID 604
UniProt ID P41182
1A-867_FC_profil značení
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human BCL6 (IG191E/A8) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1A-867_FC_Histogram
Separation of monocytes stained anti-human BCL6 (IG191E/A8) APC antibody (10 μl reagent / 100 μl of peripheral whole blood, red-filled) from monocytes stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 5 μg/ml, same as BCL6 APC concentration, black-dashed) in flow cytometry analysis (intracellular staining).

Product specific references:

Jain S, Stock A, Macian F, Putterman C: A Distinct T follicular helper cell subset infiltrates the brain in murine neuropsychiatric lupus. Front Immunol. 2018 Mar 13;9:487.
PubMed
Hoser D, Schön C, Loddenkemper C, Lohneis P, Kühl AA, Sommermann T, Blankenstein T, Willimsky G: Oncogene-specific T cells fail to eradicate lymphoma-initiating B cells in mice. Blood. 2018 Aug 30;132(9):924-934.
PubMed
Tan HX, Esterbauer R, Vanderven HA, Juno JA, Kent SJ, Wheatley AK: Inducible Bronchus-Associated Lymphoid Tissues (iBALT) Serve as Sites of B Cell Selection and Maturation Following Influenza Infection in Mice. Front Immunol. 2019 Mar 29;10:611.
PubMed
Jiang W, Wragg KM, Tan HX, Kelly HG, Wheatley AK, Kent SJ, Juno JA: Identification of murine antigen-specific T follicular helper cells using an activation-induced marker assay. J Immunol Methods. 2019 Apr;467:48-57.
PubMed
Variant
100 tests
1A-867-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

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