Exbio — Research products — Antibodies — Nucleus — Anti-ABRA1 Purified

Anti-ABRA1 Purified

Regulatory status

RUO

Antigen

ABRA1

Clone

ABRA1-01

Format

Purified

Reactivity

Human

Application

FC(IC), IP, WB

Variant

0.1 mg

11-619-C100

In stock

198.00 USD

0.025 mg

11-619-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-619-C100

In stock

198.00 USD

0.025 mg

11-619-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody ABRA1-01 recognizes human ABRA1 (Abraxas, CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kDa band on PAAGE under reducing conditions.

Application

FC(IC), IP, WB

Application details

Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining.
Western blotting: Reducing conditions.

Reactivity

Human

Immunogen

Recombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ABRA1, Abraxas 1, CCDC98, FAM175A

Antigen description

ABRA1 (Abraxas), also known as CCDC98, is an adaptor protein that is essential for formation and function of BRCA1 A tumor suppressor complex. This complex plays critical roles in DNA repair, cell cycle checkpoint control, and maintenance of genomic stability. ABRA1 mediates interaction of ubiquitin-interacting motif-containing protein RAP80 and deubiquitination enzyme BRCC36 with BRCA1/BARD1. ABRA1 controls both DNA-damage-induced formation of BRCA1 foci and BRCA1-dependent G2/M checkpoint activation.

Entrez Gene ID 84142

UniProt ID Q6UWZ7

Western blotting detection of ABRA1-EGFP fusion protein in lysate of transfected HEK293 cells. Lane A: Immunostaining with anti-ABRA1 (clone ABRA1-01) Lane B: Immunostaining with anti-EGFP (polyclonal) Lane C: Immunostaining with anti-alpha tubulin (clone TU-01)

Separation of MOLT-4 cells stained using anti-ABRA1 (ABRA1-01) purified antibody (concentration in sample 9 μg/ml, GAM APC, red-filled) from MOLT-4 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

General references:

Nikkilä J, Coleman KA, Morrissey D, Pylkäs K, Erkko H, Messick TE, Karppinen SM, Amelina A, Winqvist R, Greenberg RA: Familial breast cancer screening reveals an alteration in the RAP80 UIM domain that impairs DNA damage response function. Oncogene. 2009 Apr 23;28(16):1843-52.
PubMed

Wang B, Elledge SJ: Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage. Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20759-63.
PubMed

Liu Z, Wu J, Yu X: CCDC98 targets BRCA1 to DNA damage sites. Nat Struct Mol Biol. 2007 Aug;14(8):716-20.
PubMed

Wang B, Matsuoka S, Ballif BA, Zhang D, Smogorzewska A, Gygi SP, Elledge SJ: Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science. 2007 May 25;316(5828):1194-8.
PubMed