Anti-ABRA1 Purified

Anti-ABRA1 Purified
Regulatory status
RUO
Antigen
ABRA1
Clone
ABRA1-01
Format
Purified
Reactivity
Human
Application
FC, IP, WB
Variant
0.1 mg
11-619-C100
In stock
180.00 USD

0.025 mg
11-619-C025
Ask for delivery term HERE
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG
Specificity
The mouse monoclonal antibody ABRA1-01 recognizes human ABRA1 (Abraxas, CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kDa band on PAAGE under reducing conditions.
Application
FC, IP, WB
Application details
Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining.
Western blotting: Reducing conditions.
Reactivity
Human
Immunogen
Recombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag
Other names
ABRA1, Abraxas 1, CCDC98, FAM175A
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
ABRA1, Abraxas 1, CCDC98, FAM175A
Antigen description
ABRA1 (Abraxas), also known as CCDC98, is an adaptor protein that is essential for formation and function of BRCA1 A tumor suppressor complex. This complex plays critical roles in DNA repair, cell cycle checkpoint control, and maintenance of genomic stability. ABRA1 mediates interaction of ubiquitin-interacting motif-containing protein RAP80 and deubiquitination enzyme BRCC36 with BRCA1/BARD1. ABRA1 controls both DNA-damage-induced formation of BRCA1 foci and BRCA1-dependent G2/M checkpoint activation.
Entrez Gene ID 84142
UniProt ID Q6UWZ7
11-619 WB
Western blotting detection of ABRA1-EGFP fusion protein in lysate of transfected HEK293 cells. Lane A: Immunostaining with anti-ABRA1 (clone ABRA1-01) Lane B: Immunostaining with anti-EGFP (polyclonal) Lane C: Immunostaining with anti-alpha tubulin (clone TU-01)

General references:

Nikkilä J, Coleman KA, Morrissey D, Pylkäs K, Erkko H, Messick TE, Karppinen SM, Amelina A, Winqvist R, Greenberg RA: Familial breast cancer screening reveals an alteration in the RAP80 UIM domain that impairs DNA damage response function. Oncogene. 2009 Apr 23;28(16):1843-52.
PubMed
Wang B, Elledge SJ: Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage. Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20759-63.
PubMed
Liu Z, Wu J, Yu X: CCDC98 targets BRCA1 to DNA damage sites. Nat Struct Mol Biol. 2007 Aug;14(8):716-20.
PubMed
Wang B, Matsuoka S, Ballif BA, Zhang D, Smogorzewska A, Gygi SP, Elledge SJ: Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science. 2007 May 25;316(5828):1194-8.
PubMed
Variant
0.1 mg
11-619-C100
In stock
180.00 USD

0.025 mg
11-619-C025
Ask for delivery term HERE
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD