Exbio — Research products — Antibodies — MHC antigens — Anti-MHC Class I (α+β2m chains) PE

Anti-MHC Class I (α+β2m chains) PE

Regulatory status

RUO

Antigen

MHC Class I (α+β2m chains)

Clone

IVA26

Format

Reactivity

Mouse, Pig, Cow, Human, Rabbit, Sheep, Coturnix japonica

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-826-C100

In stock

248.60 USD

Variant

0.1 mg

1P-826-C100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody IVA26 recognizes an extracellular epitope on MHC class I (alpha + beta2m chains) expressed in various animals.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 0.5-4 μg/ml.

Reactivity

Mouse, Pig, Cow, Human, Rabbit, Sheep, Coturnix japonica

Immunogen

bovine lymphocytes

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-MHC Class I (α+β2m chains) (IVA26) PE antibody (concentration in sample 0.56 μg/ml).

Separation of human lymphocytes (red-filled) from MHC Class I negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-MHC Class I (α+β2m chains) (IVA26) PE antibody (concentration in sample 0.56 μg/ml).