Exbio — Research products — Antibodies — MHC antigens — Anti-Hu beta2-Microglobulin Purified

Anti-Hu beta2-Microglobulin Purified

Anti-Hu beta2-Microglobulin Purified
Regulatory status
RUO
Antigen
beta2-Microglobulin
Clone
B2M-02
Format
Purified
Reactivity
Pig, Human
Application
ELISA, WB, FC (QC tested), IHC-P, ICC
Variant
0.1 mg
11-456-C100
In stock
147.40 USD
0.025 mg
11-456-C025
Delivery 1 week
73.70 USD
Variant
0.1 mg
11-456-C100
In stock
147.40 USD
0.025 mg
11-456-C025
Delivery 1 week
73.70 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody B2M-02 reacts with beta2-microglobulin (beta2M), an extracellular antigen associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
Application
ELISA, WB, FC (QC tested), IHC-P, ICC
Application details
Immunohistochemistry (paraffin sections): Heat-mediated antigen retrieval; recommended dilution: 5 μg/ml; positive tissue: liver.
Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions preferred.
Flow cytometry: Recommended dilution: 1 μg/ml; positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes.
ELISA: Working dilution should be determinated by investigator.
Reactivity
Pig, Human
Immunogen
Purified isolated human beta2-microglobulin.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
B2M
Antigen description
Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Entrez Gene ID 567
UniProt ID P61769
1F-456 FC
Flow cytometry analysis (surface staining) of human peripheral blood cells by mouse monoclonal anti-beta2-microglobulin antibody B2M-02.
11-456_WB
Western blotting analysis of human β2-microglobulin using mouse monoclonal antibody B2M-02 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. β2-microglobulin was detected around 12 kDa.

Product specific references:

Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98(5):1561-6.
PubMed
Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76.
PubMed
Variant
0.1 mg
11-456-C100
In stock
147.40 USD
0.025 mg
11-456-C025
Delivery 1 week
73.70 USD
Variant
0.1 mg
11-456-C100
In stock
147.40 USD
0.025 mg
11-456-C025
Delivery 1 week
73.70 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product