Exbio — Research products — Antibodies — MHC antigens — Anti-Hu beta2-Microglobulin Purified

Anti-Hu beta2-Microglobulin Purified

Regulatory status

RUO

Antigen

beta2-Microglobulin

Clone

B2M-02

Format

Purified

Reactivity

Pig, Human

Application

ELISA, WB, FC (QC tested), IHC-P, ICC

Variant

0.1 mg

11-456-C100

In stock

143.00 USD

0.025 mg

11-456-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-456-C100

In stock

143.00 USD

0.025 mg

11-456-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody B2M-02 reacts with beta2-microglobulin (beta2M), an extracellular antigen associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

Application

ELISA, WB, FC (QC tested), IHC-P, ICC

Application details

Immunohistochemistry (paraffin sections): Heat-mediated antigen retrieval; recommended dilution: 5 μg/ml; positive tissue: liver.
Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions preferred.
Flow cytometry: Recommended dilution: 1 μg/ml; positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes.
ELISA: Working dilution should be determinated by investigator.

Reactivity

Pig, Human

Immunogen

Purified isolated human beta2-microglobulin.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

B2M

Antigen description

Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Entrez Gene ID 567

UniProt ID P61769

Flow cytometry analysis (surface staining) of human peripheral blood cells by mouse monoclonal anti-beta2-microglobulin antibody B2M-02.

Western blotting analysis of human β2-microglobulin using mouse monoclonal antibody B2M-02 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. β2-microglobulin was detected around 12 kDa.

Product specific references:

Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98(5):1561-6.
PubMed

Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76.
PubMed