Anti-Hu beta2-Microglobulin PerCP-Cy™5.5

Anti-Hu beta2-Microglobulin PerCP-Cy™5.5
Regulatory status
RUO
Antigen
beta2-Microglobulin
Clone
B2M-01
Format
PerCP-Cy™5.5
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T9-237-T100
Ask for delivery term HERE
300.00 USD

25 tests
T9-237-T025
Delivery 1 week
150.00 USD
Variant
0.1 mg
11-261-C100
In stock
300.00 USD

0.1 mg
11-261-C100
In stock
150.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody B2M-01 reacts with beta2-microglobulin (beta2M; extracellular antigen), associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Negative species
Rabbit, Dog, Mouse, Cow, Chicken
Immunogen
Purified human beta2-microglobulin
Preparation
Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy™5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
B2M
Antigen description
Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Entrez Gene ID 567
UniProt ID P61769
T9-237 FC
Flow cytometry analysis (surface staining) of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) PerCP-CyTM5.5.

Product specific references:

Poláková K, Železníková T, Russ G: HLA-G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb;37(2):139-45.
PubMed
Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA-G Expression in Human Neural Cells after Neurotropic Viral Infections. J Virol. 2005 Dec; 79(24): 15226-15237.
PubMed
Khurana M, Traum AZ, Aivado M, Wells MP, Guerrero M, Grall F, Libermann TA, Schachter AD: Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol. 2006 Sep;21(9):1257-65. Epub 2006 Jun 30.
PubMed
Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76.
PubMed
Variant
100 tests
T9-237-T100
Ask for delivery term HERE
300.00 USD

25 tests
T9-237-T025
Delivery 1 week
150.00 USD
Variant
0.1 mg
11-261-C100
In stock
300.00 USD

0.1 mg
11-261-C100
In stock
150.00 USD