Anti-Hu beta2-Microglobulin Biotin

Anti-Hu beta2-Microglobulin Biotin
Regulatory status
RUO
Antigen
beta2-Microglobulin
Clone
B2M-01
Format
Biotin
Reactivity
Human
Variant
0.1 mg
1B-237-C100
In stock
198.00 USD

0.025 mg
1B-237-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
1B-237-C100
In stock
198.00 USD

0.025 mg
1B-237-C025
Delivery 1 week
99.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody B2M-01 reacts with beta2-microglobulin (beta2M; extracellular antigen), associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
Application details
Flow cytometry: Recommended dilution: 1-2 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes.
Western blotting: Recommended dilution: 2 μg/ml; non-reducing conditions.
Reactivity
Human
Negative species
Rabbit, Dog, Mouse, Cow, Chicken
Immunogen
Purified human beta2-microglobulin
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
B2M
Antigen description
Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Entrez Gene ID 567
UniProt ID P61769
1B-237 FC
Flow cytometry analysis (surface staining) of β2-microglobulin in human peripheral blood with anti-β2-microglobulin (B2M-01) biotin.
1B-237_WB
Western blotting analysis of human β2-microglobulin using mouse monoclonal antibody B2M-01 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse monoclonal antibody followed by IRDye800-conjugated streptavidin. β2-microglobulin was detected around 12 kDa.

Product specific references:

Poláková K, Železníková T, Russ G: HLA-G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb;37(2):139-45.
PubMed
Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA-G Expression in Human Neural Cells after Neurotropic Viral Infections. J Virol. 2005 Dec; 79(24): 15226-15237.
PubMed
Khurana M, Traum AZ, Aivado M, Wells MP, Guerrero M, Grall F, Libermann TA, Schachter AD: Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol. 2006 Sep;21(9):1257-65. Epub 2006 Jun 30.
PubMed
Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76.
PubMed
Polakova K, Karpatova M, Russ G: Dissociation of beta 2-microglobulin is responsible for selective reduction of HLA class I antigenicity following acid treatment of cells. Mol Immunol. 1993 Oct;30(14):1223-30.
PubMed
Variant
0.1 mg
1B-237-C100
In stock
198.00 USD

0.025 mg
1B-237-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
1B-237-C100
In stock
198.00 USD

0.025 mg
1B-237-C025
Delivery 1 week
99.00 USD