Exbio — Research products — Antibodies — MHC antigens — Anti-HLA-G Purified

Anti-HLA-G Purified

Regulatory status

RUO

Antigen

HLA-G

Clone

G233

Format

Purified

Reactivity

Human

Application

ELISA, IP, FC (QC tested)

Variant

0.1 mg

11-494-C100

In stock

190.00 USD

0.025 mg

11-494-C025

Delivery 1 week

95.00 USD

Variant

0.1 mg

11-261-C100

In stock

190.00 USD

0.1 mg

11-261-C100

In stock

95.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody G233 recognizes an extracellular epitope on several isoforms of HLA-G expressed in all populations of extravillous trophoblast (cell columns, interstitial trophoblast, endovascular trophoblast, placental bed giant cells). HLA-G belongs to the nonclassical MHC Class I molecules (MHC Class Ib). The antibody G233 has been found not to cross-react with any other MHC Class I antigens (HLA-A, -B, -C, -E, -F).

Application

ELISA, IP, FC (QC tested)

Application details

Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

HLA-A2.1/human beta2-microglobulin double transgenic mice were immunized with murine L cells transfected with both human beta2-microglobulin and HLA-G.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography

Formulation

Phosphate buffered saline (PBS) solution with 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Entrez Gene ID 3135

UniProt ID P17693

Flow cytometry analysis (surface staining) of HLA-G in HLA-G transfectants using anti-HLA-G (G233) purified / GAM-APC.

General references:

Creput C, Durrbach A, Menier C, Guettier C, Samuel D, Dausset J, Charpentier B, Carosella ED, Rouas-Freiss N. Human leukocyte antigen-G (HLA-G) expression in biliary epithelial cells is associated with allograft acceptance in liver-kidney transplantation. J Hepatol. 2003 39(4):587-94.
PubMed

Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, LeDanff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 64(3):315-26.
PubMed

Product specific references:

Gonzalez A, Alegre E, Arroyo A, LeMaoult J, Echeveste JI: Identification of circulating nonclassic human leukocyte antigen G (HLA-G)-like molecules in exudates. Clin Chem. 2011 Jul;57(7):1013-22.
PubMed

Van Lierop MJ, Wijnands F, Loke YW, Emmer PM, Lukassen HG, Braat DD, van der Meer A, Mosselman S, Joosten I: Detection of HLA-G by a specific sandwich ELISA using monoclonal antibodies G233 and 56B. Mol Hum Reprod. 2002 Aug;8(8):776-84.
PubMed

Bulmer JN, Cioni R, Bussani C, Cirigliano V, Sole F, Costa C, Garcia P, Adinolfi M: HLA-G positive trophoblastic cells in transcervical samples and their isolation and analysis by laser microdissection and QF-PCR. Prenat Diagn. 2003 Jan;23(1):34-9.
PubMed

Loke YW, King A, Burrows T, Gardner L, Bowen M, Hiby S, Howlett S, Holmes N, Jacobs D: Evaluation of trophoblast HLA-G antigen with a specific monoclonal antibody. Tissue Antigens. 1997 Aug;50(2):135-46.
PubMed

Mandelboim O, Pazmany L, Davis DM, Vales-Gomez M, Reyburn HT, Rybalov B, Strominger JL: Multiple receptors for HLA-G on human natural killer cells. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14666-70.
PubMed

Rajagopalan S, Long EO: A human histocompatibility leukocyte antigen (HLA)-G-specific receptor expressed on all natural killer cells. J Exp Med. 1999 Apr 5;189(7):1093-100.
PubMed

Blaschitz A, Hutter H, Dohr G: HLA Class I protein expression in the human placenta. Early Pregnancy. 2001 Jan;5(1):67-9.
PubMed

Imudia AN, Suzuki Y, Kilburn BA, Yelian FD, Diamond MP, Romero R, Armant DR: Retrieval of trophoblast cells from the cervical canal for prediction of abnormal pregnancy: a pilot study. Hum Reprod. 2009 Sep;24(9):2086-92.
PubMed

Frumento G, Franchello S, Palmisano GL, Nicotra MR, Giacomini P, Loke YW, Geraghty DE, Maio M, Manzo C, Natali PG, Ferrara GB. Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment. Tissue Antigens. 2000 Jul;56(1):30-7.
PubMed

Variant

0.1 mg

11-494-C100

In stock

190.00 USD

0.025 mg

11-494-C025

Delivery 1 week

95.00 USD

Variant

0.1 mg

11-261-C100