Exbio — Research products — Antibodies — MHC antigens — Anti-HLA-G Biotin

Anti-HLA-G Biotin

Regulatory status

RUO

Antigen

HLA-G

Clone

5A6G7

Format

Biotin

Reactivity

Human

Application

ELISA, WB, FC (QC tested), IHC(F), IHC(P), ICC

Variant

0.1 mg

1B-431-C100

In stock

260.00 USD

0.025 mg

1B-431-C025

Delivery 1 week

130.00 USD

Variant

0.1 mg

11-261-C100

In stock

260.00 USD

0.1 mg

11-261-C100

In stock

130.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allows to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.

Application

ELISA, WB, FC (QC tested), IHC(F), IHC(P), ICC

Application details

Flow cytometry: Recommended dilution: 1-3 μg/ml.

Reactivity

Human

Immunogen

C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Entrez Gene ID 3135

UniProt ID P17693

Flow cytometry analysis (intracellular staining) of HLA-G in HLA-G1 transfectants with anti-HLA-G (5A6G7) biotin / streptavidin-APC.

General references:

Creput C, Durrbach A, Menier C, Guettier C, Samuel D, Dausset J, Charpentier B, Carosella ED, Rouas-Freiss N. Human leukocyte antigen-G (HLA-G) expression in biliary epithelial cells is associated with allograft acceptance in liver-kidney transplantation. J Hepatol. 2003 39(4):587-94.
PubMed

Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, LeDanff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 64(3):315-26.
PubMed

Product specific references:

Poláková K, Železníková T, Russ G: HLA-G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb;37(2):139-45.
PubMed

Rebmann V, Lemaoult J, Rouas-Freiss N, Carosella ED, Grosse-Wilde H: Report of the Wet Workshop for Quantification of Soluble HLA-G in Essen, 2004. Hum Immunol. 2005 Aug;66(8):853-63.
PubMed

Le Rond S, Le Maoult J, Creput C, Menier C, Deschamps M, Le Friec G, Amiot L, Durrbach A, Dausset J, Carosella ED, Rouas-Freiss N: Alloreactive CD4+ and CD8+ T cells express the immunotolerant HLA-G molecule in mixed lymphocyte reactions: in vivo implications in transplanted patients. Eur J Immunol. 2004 Mar;34(3):649-60.
PubMed

Le Rond S, Gonzalez A, Gonzalez AS, Carosella ED, Rouas-Freiss N: Indoleamine 2,3 dioxygenase and human leucocyte antigen-G inhibit the T-cell alloproliferative response through two independent pathways. Immunology. 2005 Nov; 116(3): 297-307.
PubMed

Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA-G Expression in Human Neural Cells after Neurotropic Viral Infections. J Virol. 2005 Dec; 79(24): 15226-15237.
PubMed

Variant

0.1 mg

1B-431-C100

In stock

260.00 USD

0.025 mg

1B-431-C025

Delivery 1 week

130.00 USD

Variant

0.1 mg

11-261-C100