Anti-HLA-F Purified

Anti-HLA-F Purified
Regulatory status
RUO
Antigen
HLA-F
Clone
3D11
Format
Purified
Reactivity
Human
Application
WB, FC (QC tested)
Variant
0.1 mg
11-823-C100
In stock
Variant
0.1 mg
11-261-C100
In stock
Product details
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 3D11 recognizes HLA-F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.
Application
WB, FC (QC tested)
Reactivity
Human
Immunogen
Inclusion body-derived HLA-F heavy chain
Other names
CDA12
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

Product specific references:

Lee N, Geraghty DE: HLA-F surface expression on B cell and monocyte cell lines is partially independent from tapasin and completely independent from TAP. J Immunol. 2003 Nov 15;171(10):5264-71.
PubMed
Lee N, Ishitani A, Geraghty DE: HLA-F is a surface marker on activated lymphocytes. Eur J Immunol. 2010 Aug;40(8):2308-18.
PubMed
Goodridge JP, Burian A, Lee N, Geraghty DE: HLA-F and MHC class I open conformers are ligands for NK cell Ig-like receptors. J Immunol. 2013 Oct 1;191(7):3553-62.
PubMed
Burian A, Wang KL, Finton KA, Lee N, Ishitani A, Strong RK, Geraghty DE: HLA-F and MHC-I Open Conformers Bind Natural Killer Cell Ig-Like Receptor KIR3DS1. PLoS One. 2016 Sep 20;11(9):e0163297.
PubMed
Variant
0.1 mg
11-823-C100
In stock
Variant
0.1 mg
11-261-C100
In stock