Exbio — Research products — Antibodies — MHC antigens — Anti-HLA-F PE

Anti-HLA-F PE

Regulatory status

RUO

Antigen

HLA-F

Clone

3D11

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-823-C100

In stock

352.00 USD

Variant

0.1 mg

1P-823-C100

In stock

352.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 3D11 recognizes an extracellular epitope of HLA-F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml; extracellular and intracellular staining.

Reactivity

Human

Immunogen

Inclusion body-derived HLA-F heavy chain

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CDA12

Antigen description

HLA-F, an MHC class I molecule, is a type I transmembrane protein (heavy chain), which forms heterodimers with beta-2 microglobulin (light chain) and binds to KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, and TAP. Unlike most other HLA proteins, HLA-F is mainly localized in the endoplasmic reticulum and Golgi apparatus, with only a small amount present on the cell surface in some cell types; surface expression can be induced by cell activation. It is thought to bind a restricted subset of peptides for immune presentation. Multiple transcript variants encoding different isoforms have been found for HLA-F gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.

Entrez Gene ID 3134

UniProt ID P30511

Flow cytometry multicolor intracellular staining pattern of human lymphocytes stained using anti-human CD20 (2H7) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/ml, red) or mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as anti-HLA-F PE concentration, black).

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD20 (2H7) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/ml, red) or mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as anti-HLA-F PE concentration, black).

Separation of lymphocytes stained using anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/ml, red-filled) from lymphocytes stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as anti-HLA-F PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of human stimulated (PMA + Ionomycin) whole blood cells.

Separation of lymphocytes stained using anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/ml, red-filled) from lymphocytes stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as anti-HLA-F PE concentration, black-dashed) in flow cytometry analysis (surface staining) of human stimulated (PMA + Ionomycin) whole blood cells.

Product specific references:

Lee N, Geraghty DE: HLA-F surface expression on B cell and monocyte cell lines is partially independent from tapasin and completely independent from TAP. J Immunol. 2003 Nov 15;171(10):5264-71.
PubMed

Lee N, Ishitani A, Geraghty DE: HLA-F is a surface marker on activated lymphocytes. Eur J Immunol. 2010 Aug;40(8):2308-18.
PubMed

Goodridge JP, Burian A, Lee N, Geraghty DE: HLA-F and MHC class I open conformers are ligands for NK cell Ig-like receptors. J Immunol. 2013 Oct 1;191(7):3553-62.
PubMed

Burian A, Wang KL, Finton KA, Lee N, Ishitani A, Strong RK, Geraghty DE: HLA-F and MHC-I open conformers bind natural killer cell Ig-like receptor KIR3DS1. PLoS One. 2016 Sep 20;11(9):e0163297.
PubMed