Exbio — Research products — Antibodies — MHC antigens — Anti-HLA-E Purified

Anti-HLA-E Purified

Regulatory status

RUO

Antigen

HLA-E

Clone

MEM-E/02

Format

Purified

Reactivity

Human, Non-human primates

Application

WB, IHC(P)

Variant

0.1 mg

11-361-C100

In stock

187.00 USD

0.025 mg

11-361-C025

In stock

93.50 USD

Variant

0.1 mg

11-361-C100

In stock

187.00 USD

0.025 mg

11-361-C025

In stock

93.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-E/02 specifically reacts with an extracellular epitope on denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types.

Application

WB, IHC(P)

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive control: human placenta; heat antigen retrieval (sodium citrate), incubation with mAb: 1 hour / RT, detection DAB.

Reactivity

Human, Non-human primates

Immunogen

Recombinant human HLA-E denaturated heavy chain.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E

Antigen description

HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).

Entrez Gene ID 3133

UniProt ID P13747

Anti-Hu HLA-E purified antibody (clone MEM-E/02) specificity verification by flow cytometry

Anti-Hu HLA-E purified antibody (concentration in sample 5 μg/ml, red-filled histogram) binds specifically to surface HLA-E on surface of HLA-E transfected LCL 721.221 cells, but not to RAJI cells, K562 cells, HLA-G transfected LCL 721.221 cells and non-transfected LCL 721.221 cells.
Level of non-specific binding was assessed using Mouse IgG1 isotype control PE (MOPC-21) under same conditions (concentration in sample 5 μg/ml, black-dashed histogram).

Product specific references:

Spaans VM, Peters AA, Fleuren GJ, Jordanova ES: HLA-E expression in cervical adenocarcinomas: association with improved long-term survival. J Transl Med. 2012 Sep 4;10:184.
PubMed

Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep;13(9):822-30.
PubMed

Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64(3):315-26.
PubMed

Dambaeva SV, Bondarenko GI, Grendell RL, Kravitz RH, Durning M, Golos TG: Non-classical MHC-E (Mamu-E) expression in the rhesus monkey placenta. Placenta. 2008 Jan;29(1):58-70.
PubMed