Anti-HLA-E Purified

Anti-HLA-E Purified
Regulatory status
RUO
Antigen
HLA-E
Clone
3D12
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-824-C100
In stock
187.00 USD
Variant
0.1 mg
11-824-C100
In stock
187.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 3D12 (also known as 3D12HLA-E) recognizes native extracellular part of HLA-E, an ubiquitously expressed non-classical MHC class I molecule, as well as free HLA-E.
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Human
Immunogen
recombinant human HLA-E
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E
Antigen description
HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).
Entrez Gene ID 3133
UniProt ID P13747
11-824_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human HLA-E (3D12) purified antibody (concentration in sample 4 μg/ml) GAM APC.
11-824_FC_Histogram
Separation of human lymphocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-E (3D12) purified antibody (concentration in sample 4 μg/ml) GAM APC.

Product specific references:

Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep;13(9):822-30.
PubMed
Tremante E, Lo Monaco E, Ingegnere T, Sampaoli C, Fraioli R, Giacomini P: Monoclonal antibodies to HLA-E bind epitopes carried by unfolded β2 m-free heavy chains. Eur J Immunol. 2015 Aug;45(8):2356-64.
PubMed
Ravindranath MH, Pham T, El-Awar N, Kaneku H, Terasaki PI: Anti-HLA-E mAb 3D12 mimics MEM-E/02 in binding to HLA-B and HLA-C alleles: Web-tools validate the immunogenic epitopes of HLA-E recognized by the antibodies. Mol Immunol. 2011 Jan;48(4):423-30.
PubMed
Sensi M, Pietra G, Molla A, Nicolini G, Vegetti C, Bersani I, Millo E, Weiss E, Moretta L, Mingari MC, Anichini A: Peptides with dual binding specificity for HLA-A2 and HLA-E are encoded by alternatively spliced isoforms of the antioxidant enzyme peroxiredoxin 5. Int Immunol. 2009 Mar;21(3):257-68.
PubMed
McMurtrey C, Harriff MJ, Swarbrick GM, Duncan A, Cansler M, Null M, Bardet W, Jackson KW, Lewinsohn DA, Hildebrand W, Lewinsohn DM: T cell recognition of Mycobacterium tuberculosis peptides presented by HLA-E derived from infected human cells. PLoS One. 2017 Nov 27;12(11):e0188288.
PubMed
Lee N, Llano M, Carretero M, Ishitani A, Navarro F, López-Botet M, Geraghty DE: HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A. Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5199-204.
PubMed
Variant
0.1 mg
11-824-C100
In stock
187.00 USD
Variant
0.1 mg
11-824-C100
In stock
187.00 USD

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