Anti-HLA-E Purified

Anti-HLA-E Purified
Regulatory status
RUO
Antigen
HLA-E
Clone
3D12
Format
Purified
Reactivity
Human
Application
FC (QC tested)
Variant
0.1 mg
11-824-C100
In stock
Variant
0.1 mg
11-261-C100
In stock
Product details
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 3D12 (also known as 3D12HLA-E) recognizes native extracellular part of HLA-E, an ubiquitously expressed non-classical MHC class I molecule, as well as free HLA-E.
Application
FC (QC tested)
Reactivity
Human
Other names
HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E
Concentration
1 mg/ml
Preparation
Purified from cell culture supernatant by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

Product specific references:

Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep;13(9):822-30.
PubMed
Tremante E, Lo Monaco E, Ingegnere T, Sampaoli C, Fraioli R, Giacomini P: Monoclonal antibodies to HLA-E bind epitopes carried by unfolded β2 m-free heavy chains. Eur J Immunol. 2015 Aug;45(8):2356-64.
PubMed
Ravindranath MH, Pham T, El-Awar N, Kaneku H, Terasaki PI: Anti-HLA-E mAb 3D12 mimics MEM-E/02 in binding to HLA-B and HLA-C alleles: Web-tools validate the immunogenic epitopes of HLA-E recognized by the antibodies. Mol Immunol. 2011 Jan;48(4):423-30.
PubMed
Sensi M, Pietra G, Molla A, Nicolini G, Vegetti C, Bersani I, Millo E, Weiss E, Moretta L, Mingari MC, Anichini A: Peptides with dual binding specificity for HLA-A2 and HLA-E are encoded by alternatively spliced isoforms of the antioxidant enzyme peroxiredoxin 5. Int Immunol. 2009 Mar;21(3):257-68.
PubMed
McMurtrey C, Harriff MJ, Swarbrick GM, Duncan A, Cansler M, Null M, Bardet W, Jackson KW, Lewinsohn DA, Hildebrand W, Lewinsohn DM: T cell recognition of Mycobacterium tuberculosis peptides presented by HLA-E derived from infected human cells. PLoS One. 2017 Nov 27;12(11):e0188288.
PubMed
Lee N, Llano M, Carretero M, Ishitani A, Navarro F, López-Botet M, Geraghty DE: HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A. Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5199-204.
PubMed
Variant
0.1 mg
11-824-C100
In stock
Variant
0.1 mg
11-261-C100
In stock