Anti-HLA-E Purified

Anti-HLA-E Purified
Regulatory status
RUO
Antigen
HLA-E
Clone
MEM-E/07
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-397-C100
In stock
170.00 USD

0.025 mg
11-397-C025
Delivery 1 week
85.00 USD
Variant
0.1 mg
11-261-C100
In stock
170.00 USD

0.1 mg
11-261-C100
In stock
85.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody MEM-E/07 recognizes an extracellular epitope on native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen.
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Human
Immunogen
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E
Antigen description
HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).
Entrez Gene ID 3133
UniProt ID P13747
11-397_IHC
Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/07). Commercially tested by LifeSpan BioSciences.

Product specific references:

Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le VT, Galun E, Horejsi V, Isakov O, Shomron N, Wolf DG, Hengel H, Mandelboim O: MicroRNA editing facilitates immune elimination of HCMV infected cells. PLoS Pathog. 2014 Feb 27;10(2):e1003963.
PubMed
Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep;13(9):822-30.
PubMed
Palmisano GL, Contardi E, Morabito A, Gargaglione V, Ferrara GB, Pistillo MP.: HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol. 2005 Jan;66(1):1-12.
PubMed
Sibilio L. et al., Tissue Antigens 62, 339, 2003. (Abstract 45 from the 3rd Int. Conference on HLA-G, Paris, July 2003).
Variant
0.1 mg
11-397-C100
In stock
170.00 USD

0.025 mg
11-397-C025
Delivery 1 week
85.00 USD
Variant
0.1 mg
11-261-C100
In stock
170.00 USD

0.1 mg
11-261-C100
In stock
85.00 USD

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