Anti-HLA-DR Purified

Anti-HLA-DR Purified
Regulatory status
RUO
Antigen
HLA-DR
Clone
MEM-12
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-474-C100
In stock
140.00 USD

0.025 mg
11-474-C025
Delivery 1 week
70.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD

0.1 mg
11-261-C100
In stock
70.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody MEM-12 recognizes a common extracellular epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
Application details
Flow cytometry: Recommended dilution: 2 μg/ml; positive control: B-lymphocytes, monocytes, DAUDI cell line, RAJI cell line.
Western blotting and Immunoprecipitation: Positive control: B-lymphocytes, monocytes, DAUDI cell line, RAJI cell line; non-reducing conditions.
Reactivity
Human
Immunogen
thymocyte membrane
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Antigen description
HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.
11-474_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR (MEM-12) purified antibody (concentration in sample 0,3 μg/ml, GAM APC).
11-474_FC_Histogram
Separation of human monocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human HLA-DR (MEM-12) purified antibody (concentration in sample 0,3 μg/ml, GAM APC).

Product specific references:

Horejsi V, Nemec M, Angelisova P, Kristofova H, Gorga JC, Hilgert I: Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens. 1986 Nov;28(5):288-97.
PubMed
Variant
0.1 mg
11-474-C100
In stock
140.00 USD

0.025 mg
11-474-C025
Delivery 1 week
70.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD

0.1 mg
11-261-C100
In stock
70.00 USD

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