Exbio — Research products — Antibodies — MHC antigens — Anti-HLA-DR FITC

Anti-HLA-DR FITC

Regulatory status

RUO

Antigen

HLA-DR

Clone

MEM-12

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-474-T100

In stock

198.00 USD

25 tests

1F-474-T025

In stock

99.00 USD

Variant

100 tests

1F-474-T100

In stock

198.00 USD

25 tests

1F-474-T025

In stock

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-12 recognizes a common extracellular epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

thymocyte membrane

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR (MEM-12) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human HLA-DR (MEM-12) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human HLA-DR positive CD3 negative lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-DR (MEM-12) APC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Horejsi V, Nemec M, Angelisova P, Kristofova H, Gorga JC, Hilgert I: Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens. 1986 Nov;28(5):288-97.
PubMed

Sochorová K, Budínský V, Rožková D, Tobiášová Z, Dusilová-Sulková S, Spíšek R, Bartůňková J: Paricalcitol (19-nor-1,25-dihydroxyvitamin D2) and calcitriol (1,25-dihydroxyvitamin D3) exert potent immunomodulatory effects on dendritic cells and inhibit induction of antigen-specific T cells. Clin Immunol. 2009 Oct;133(1):69-77.
PubMed