Anti-HLA-DR FITC

Anti-HLA-DR FITC
Regulatory status
RUO
Antigen
HLA-DR
Clone
MEM-12
Format
FITC
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1F-474-T100
In stock
180.00 USD

25 tests
1F-474-T025
Out of stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody MEM-12 recognizes a common extracellular epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
thymocyte membrane
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1F-474_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR (MEM-12) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).
1F-474_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human HLA-DR (MEM-12) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1F-474_FC_Histogram
Separation of human HLA-DR positive CD3 negative lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-DR (MEM-12) APC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Horejsi V, Nemec M, Angelisova P, Kristofova H, Gorga JC, Hilgert I: Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens. 1986 Nov;28(5):288-97.
PubMed
Variant
100 tests
1F-474-T100
In stock
180.00 USD

25 tests
1F-474-T025
Out of stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD

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