Anti-HLA-DR/DP Purified

Anti-HLA-DR/DP Purified
Regulatory status
RUO
Antigen
HLA-DR/DP
Clone
HL-38
Format
Purified
Reactivity
Pig, Human
Variant
0.1 mg
11-239-C100
In stock
143.00 USD

0.025 mg
11-239-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-239-C100
In stock
143.00 USD

0.025 mg
11-239-C025
Delivery 1 week
71.50 USD
Product details
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The antibody HL-38 recognizes an extracellular common epitope on beta-chain of human HLA-DR and HLA-DP. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
Application details
Western blotting: Recommended dilution: 1 µg/ml
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Pig, Human
Immunogen
Raji Burkitt's lymphoma cell line
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-239_IHC
Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-HLA-DR+DP (HL-38). Commercially tested by LifeSpan BioSciences.
11-239_WB_appl
Anti-HLA-DR/DP (clone HL-38) works in WB application under reducing and non-reducing conditions.

Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis.

Nitrocellulose membrane blot was probed with mouse IgG2a monoclonal antibody HL-38 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.

HLA-DR/DP molecules were detected at ~25 kDa in Raji cell line.
11-239 FC
Flow cytometry analysis (surface staining) of human peripheral blood with anti-HLA-DR+DP (HL-38) PE.

Product specific references:

Horejsi V, Nemec M, Angelisova P, Kristofova H, Gorga JC, Hilgert I: Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens. 1986 Nov;28(5):288-97.
PubMed
Variant
0.1 mg
11-239-C100
In stock
143.00 USD

0.025 mg
11-239-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-239-C100
In stock
143.00 USD

0.025 mg
11-239-C025
Delivery 1 week
71.50 USD

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