Anti-HLA-DR/DP Purified

Anti-HLA-DR/DP Purified
Regulatory status
RUO
Antigen
HLA-DR/DP
Clone
MEM-136
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-296-C100
In stock
143.00 USD

0.025 mg
11-296-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-296-C100
In stock
143.00 USD

0.025 mg
11-296-C025
Delivery 1 week
71.50 USD
Product details
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody MEM-136 recognizes a common extracellular epitope on beta-chain of human HLA-DR and HLA-DP. It reacts with alpha/beta dimer as well as with dissociated beta-subunit. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Western blotting: Recommended dilution: 1 μg/ml.
Reactivity
Human
Immunogen
PHA-activated peripheral blood lymphocytes.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-296_FC_Histogram
Separation of human HLA-DR/DP positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-DR/DP (MEM-136) purified antibody (concentration in sample 4 μg/ml) GAM APC.
11-296_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR/DP (MEM-136) purified antibody (concentration in sample 4 μg/ml) GAM APC.
11-296_IHC
Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-HLA-DR+DP (MEM-136). Commercially tested by LifeSpan BioSciences.
11-296_WB_appl
Anti-HLA-DR/DP (clone MEM-136) works in WB application under reducing and non-reducing conditions.

Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody MEM-136 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.

HLA-DR/DP molecules were detected at ~25 kDa in Raji cell line.
11-296 WB
Western blotting analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136). The antibody MEM-136 recognizes β-chain of HLA-DR antigen under non-reducing conditions. Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06; 11-457-C100)

Product specific references:

Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 27;9:852.
PubMed
Koch C, Staffler G, Huttinger R, Hilgert I, Prager E, Cerny J, Steinlein P, Majdic O, Horejsi V, Stockinger H: T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol. 1999 May;11(5):777-86.
PubMed
Variant
0.1 mg
11-296-C100
In stock
143.00 USD

0.025 mg
11-296-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-296-C100
In stock
143.00 USD

0.025 mg
11-296-C025
Delivery 1 week
71.50 USD

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