Exbio — Research products — Antibodies — MHC antigens — Anti-HLA-DR/DP Purified

Anti-HLA-DR/DP Purified

Regulatory status

RUO

Antigen

HLA-DR/DP

Clone

HL-38

Format

Purified

Reactivity

Pig, Human

Application

IP, WB, FC (QC tested), IHC-P

Variant

0.1 mg

11-239-C100

In stock

143.00 USD

0.025 mg

11-239-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-239-C100

In stock

143.00 USD

0.025 mg

11-239-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody HL-38 recognizes an extracellular common epitope on beta-chain of human HLA-DR and HLA-DP. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).

Application

IP, WB, FC (QC tested), IHC-P

Application details

Western blotting: Recommended dilution: 1 µg/ml
Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Pig, Human

Immunogen

Raji Burkitt's lymphoma cell line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-HLA-DR+DP (HL-38). Commercially tested by LifeSpan BioSciences.

Anti-HLA-DR/DP (clone HL-38) works in WB application under reducing and non-reducing conditions.

Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG2a monoclonal antibody HL-38 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.

HLA-DR/DP molecules were detected at ~25 kDa in Raji cell line.

Flow cytometry analysis (surface staining) of human peripheral blood with anti-HLA-DR+DP (HL-38) PE.

Product specific references:

Horejsi V, Nemec M, Angelisova P, Kristofova H, Gorga JC, Hilgert I: Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens. 1986 Nov;28(5):288-97.
PubMed