Anti-HLA-DR/DP PE

Anti-HLA-DR/DP PE
Regulatory status
RUO
Antigen
HLA-DR/DP
Clone
MEM-136
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-296-T100
In stock
242.00 USD

25 tests
1P-296-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-296-T100
In stock
242.00 USD

25 tests
1P-296-T025
Delivery 1 week
121.00 USD
Product details
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody MEM-136 recognizes a common extracellular epitope on beta-chain of human HLA-DR and HLA-DP. It reacts with alpha/beta dimer as well as with dissociated beta-subunit. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
PHA-activated peripheral blood lymphocytes.
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1P-296_FC_CD Maps-profil značení
Anti-human HLA-DR/DP PE antibody (clone MEM-136) works in flow cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Mouse monoclonal anti-human HLA-DR/DP PE antibody (clone MEM-136) was used in amount of 20 µl in 100 µl of blood sample (2 x 106 cells).
1P-296_FC_CD Maps-histogram
Expression profiling on peripheral blood subsets using Anti-human HLA-DR/DP PE antibody (clone MEM-136).

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 106 cells) was added to the mixture of anti-human HLA-DR/DP PE antibody (clone MEM-136, 20 μl reagent / 100 μl of stained blood sample) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Next, optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) were added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Product specific references:

Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 27;9:852.
PubMed
Koch C, Staffler G, Huttinger R, Hilgert I, Prager E, Cerny J, Steinlein P, Majdic O, Horejsi V, Stockinger H: T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol. 1999 May;11(5):777-86.
PubMed
Variant
100 tests
1P-296-T100
In stock
242.00 USD

25 tests
1P-296-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-296-T100
In stock
242.00 USD

25 tests
1P-296-T025
Delivery 1 week
121.00 USD

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