Anti-HLA-DR Alexa Fluor® 700

Anti-HLA-DR Alexa Fluor<sup>®</sup> 700
Regulatory status
RUO
Antigen
HLA-DR
Clone
MEM-12
Format
Alexa Fluor® 700
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
A7-474-T100
In stock
242.00 USD

25 tests
A7-474-T025
Delivery 1 week
121.00 USD
Variant
100 tests
A7-474-T100
In stock
242.00 USD

25 tests
A7-474-T025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody MEM-12 recognizes a common extracellular epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
thymocyte membrane
Preparation
Purified antibody is conjugated with Alexa Fluor® 700 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Antigen description
HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.
A7-474_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR (MEM-12) Alexa Fluor® 700 antibody (4 μl reagent / 100 μl of peripheral whole blood).
A7-474_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human HLA-DR (MEM-12) Alexa Fluor® 700 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood
A7-474_FC_Histogram
Separation of human HLA-DR positive CD3 negative lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-DR (MEM-12) Alexa Fluor® 700 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Horejsi V, Nemec M, Angelisova P, Kristofova H, Gorga JC, Hilgert I: Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens. 1986 Nov;28(5):288-97.
PubMed
Sochorová K, Budínský V, Rožková D, Tobiášová Z, Dusilová-Sulková S, Spíšek R, Bartůňková J: Paricalcitol (19-nor-1,25-dihydroxyvitamin D2) and calcitriol (1,25-dihydroxyvitamin D3) exert potent immunomodulatory effects on dendritic cells and inhibit induction of antigen-specific T cells. Clin Immunol. 2009 Oct;133(1):69-77.
PubMed
Variant
100 tests
A7-474-T100
In stock
242.00 USD

25 tests
A7-474-T025
Delivery 1 week
121.00 USD
Variant
100 tests
A7-474-T100
In stock
242.00 USD

25 tests
A7-474-T025
Delivery 1 week
121.00 USD

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