Exbio — Research products — Antibodies — MHC antigens — Anti-HLA Class I Purified

Anti-HLA Class I Purified

Regulatory status

RUO

Antigen

HLA Class I

Clone

MEM-81

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-243-C100

In stock

143.00 USD

0.025 mg

11-243-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-243-C100

In stock

143.00 USD

0.025 mg

11-243-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-81 reacts with an extracellular epitope on human classical MHC Class I molecules in native cell-surface forms. MHC Class I molecules (MHC Class Ia) are expressed on the surface of human nucleated cell types.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Human

Immunogen

Leukocytes of a patient suffering with LGL type leukemia.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-HLA Class I (MEM-81) purified antibody (concentration in sample 1 μg/ml) GAM APC.

Separation of human leukocytes stained using anti-HLA Class I (MEM-81) purified antibody (concentration in sample 1 μg/ml, GAM APC, red-filled) from human leukocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Valenzuela NM, Mulder A, Reed EF: HLA class I antibodies trigger increased adherence of monocytes to endothelial cells by eliciting an increase in endothelial P-selectin and, depending on subclass, by engaging FcγRs. J Immunol. 2013 Jun 15;190(12):6635-50.
PubMed