Exbio — Research products — Antibodies — MHC antigens — Anti-HLA Class I PE

Anti-HLA Class I PE

Regulatory status

RUO

Antigen

HLA Class I

Clone

MEM-123

Format

Reactivity

Cow, Human, Non-human primates

Application

FC (QC tested)

Excitation laser

blue (488 nm)

test1 True

Variant

0.1 mg

1P-363-C100

In stock

293.70 USD

0.025 mg

1P-363-C025

In stock

146.85 USD

Variant

0.1 mg

1P-363-C100

In stock

293.70 USD

0.025 mg

1P-363-C025

In stock

146.85 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG3

Specificity

The antibody MEM-123 reacts with an extracellular epitope of all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. The antibody MEM-123 completely blocks binding of classical W6/32 to surface-expressed HLA-G, but does not cross-blocks the antibody MEM-G/9.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 2-4 μg/ml.

Reactivity

Cow, Human, Non-human primates

Immunogen

COS-7 African green monkey kidney cells

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-HLA Class I (MEM-123) PE antibody (concentration in sample 1.67 μg/ml).

Separation of human lymphocytes (red-filled) from HLA Class I negative CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-HLA Class I (MEM-123) PE antibody (concentration in sample 1.67 μg/ml).

Separation of human lymphocytes (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood spiked with K562 cells stained using anti-HLA Class I (MEM-123) PE antibody (concentration in sample 1.67 μg/ml).

Flow cytometry surface staining pattern of human peripheral whole blood spiked with K562 cells stained using anti-HLA Class I (MEM-123) PE antibody (concentration in sample 1.67 μg/ml).