Exbio — Research products — Antibodies — MHC antigens — Anti-HLA Class I FITC

Anti-HLA Class I FITC

Regulatory status

RUO

Antigen

HLA Class I

Clone

W6/32

Format

FITC

Reactivity

Cow, Non-human primates, Human, Cat

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1F-422-C100

In stock

260.00 USD

0.025 mg

1F-422-C025

Delivery 1 week

130.00 USD

Variant

0.1 mg

1F-422-C100

In stock

260.00 USD

0.025 mg

1F-422-C025

Delivery 1 week

130.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-3 μg/ml.

Reactivity

Cow, Non-human primates, Human, Cat

Negative species

Rabbit

Immunogen

Membrane of human tonsil cells

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA Class I (W6/32) FITC antibody (concentration in sample 3 μg/ml).

Separation of human leukocytes (red-filled) from HLA Class I negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA Class I (W6/32) FITC antibody (concentration in sample 3 μg/ml).

Product specific references:

Kievits F, Ivanyi P: Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin.Hum Immunol. 1987 Oct;20(2):115-26.</li>
PubMed

Ladasky JJ, Shum BP, Canavez F, Seuanez HN, Parham P: Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody. Immunogenetics. 1999 Apr;49(4):312-20. </li>
PubMed

Le Discorde M, Moreau P, Sabatier P, Legeais JM, Carosella ED: Expression of HLA-G in human cornea, an immune-privileged tissue. Hum Immunol. 2003 Nov;64(11):1039-44.
PubMed

Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics. 2001 Aug;53(6):440-6.
PubMed

Shields MJ, Ribaudo RK: Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin. Tissue Antigens 1998 May;51(5):567-70.
PubMed

Jacobsen, C. N. et al. (1993): Reactivities of 20 anti-human monclonal antibodies with leucocytes from ten different animal species. Vet. Immunopathol. 39: 461 - 466.
PubMed

Stern, P. et al. (1987): Class I-like MHC molecules expressed by baboon placental synctiotrophoblast. Journal of Immunology. 138 (4): 1088 - 1091.
PubMed

Neefjes, J.J. et al. (1986): A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics 23: 341-347.
PubMed

Brodsky, F.M. et al. (1982): Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics 15: 151-166.
PubMed

Barnstable, C. J., et al. (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - new tools for genetic analysis. Cell. 14: 9 - 20.
PubMed