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Exbio
—
Research products
—
Antibodies
—
MHC antigens
—
Anti-HLA-ABCE Purified
Anti-HLA-ABCE Purified
Regulatory status
RUO
Antigen
HLA-ABCE
Clone
TP25.99SF
Format
Purified
Reactivity
Human
Application
WB, FC (QC tested), IHC(F)
Variant
0.1 mg
11-813-C100
In stock
170.00 USD
0.025 mg
11-813-C025
Delivery 1 week
85.00 USD
Variant
0.1 mg
11-813-C100
In stock
170.00 USD
0.025 mg
11-813-C025
Delivery 1 week
85.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody TP25.99SF recognizes an extracellular epitope on HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens.
Application
WB, FC (QC tested), IHC(F)
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Human
Immunogen
IFN-gamma-treated human melanoma cells Colo 38
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Antigen description
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.
Flow cytometry analysis (surface staining) of human peripheral blood cells using anti-HLA-ABCE (TP25.99SF) purified, GAM-APC.
Anti-HLA-ABCE Purified (clone TP25.99SF) works in WB application.
Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of JAR, JEG3, Raji, and K562 cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 10% Tris-glycine SDS gel electrophoresis.
Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody TP25.99SF (2 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse IgG1 anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.
HLA-ABCE molecules were detected at ~40 kDa in JAR and Raji cell lines.
Product specific references:
Tanabe M, Sekimata M, Ferrone S, Takiguchi M: Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain. J Immunol. 1992 May 15;148(10):3202-9.
PubMed
Desai SA, Wang X, Noronha EJ, Zhou Q, Rebmann V, Grosse-Wilde H, Moy FJ, Powers R, Ferrone S: Structural relatedness of distinct determinants recognized by monoclonal antibody TP25.99 on beta 2-microglobulin-associated and beta 2-microglobulin-free HLA class I heavy chains. J Immunol. 2000 Sep 15;165(6):3275-83.
PubMed
Perosa F, Luccarelli G, Prete M, Favoino E, Ferrone S, Dammacco F: Beta 2-microglobulin-free HLA class I heavy chain epitope mimicry by monoclonal antibody HC-10-specific peptide. J Immunol. 2003 Aug 15;171(4):1918-26.
PubMed
Moy FJ, Desai SA, Wang X, Noronha EJ, Zhou Q, Ferrone S, Powers R: Analysis by NMR spectroscopy of the structural homology between the linear and the cyclic peptide recognized by anti-human leukocyte antigen class I monoclonal antibody TP25.99. J Biol Chem. 2000 Aug 11;275(32):24679-85.
PubMed
Blaschitz A, Hutter H, Leitner V, Pilz S, Wintersteiger R, Dohr G, Sedlmayr P: Reaction patterns of monoclonal antibodies to HLA-G in human tissues and on cell lines: a comparative study. Hum Immunol. 2000 Nov;61(11):1074-85.
PubMed
Paul P, Rouas-Freiss N, Moreau P, Cabestre FA, Menier C, Khalil-Daher I, Pangault C, Onno M, Fauchet R, Martinez-Laso J, Morales P, Villena AA, Giacomini P, Natali PG, Frumento G, Ferrara GB, McMaster M, Fisher S, Schust D, Ferrone S, Dausset J, Geraghty D, Carosella ED: HLA-G, -E, -F preworkshop: tools and protocols for analysis of non-classical class I genes transcription and protein expression. Hum Immunol. 2000 Nov;61(11):1177-95.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-813-C100
In stock
170.00 USD
0.025 mg
11-813-C025
Delivery 1 week
85.00 USD
Variant
0.1 mg
11-813-C100
In stock
170.00 USD
0.025 mg
11-813-C025
Delivery 1 week
85.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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