Exbio — Research products — Antibodies — MHC antigens — Anti-HLA-ABCE Purified

Anti-HLA-ABCE Purified

Regulatory status

RUO

Antigen

HLA-ABCE

Clone

TP25.99SF

Format

Purified

Reactivity

Human

Application

WB, FC (QC tested), IHC-F

Variant

0.1 mg

11-813-C100

In stock

192.50 USD

0.025 mg

11-813-C025

Delivery 1 week

96.25 USD

Variant

0.1 mg

11-813-C100

In stock

192.50 USD

0.025 mg

11-813-C025

Delivery 1 week

96.25 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody TP25.99SF recognizes an extracellular epitope on HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens.

Application

WB, FC (QC tested), IHC-F

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.
Western blotting: Recommended dilution: 1-2 µg/ml; non-reducing conditions.

Reactivity

Human

Immunogen

IFN-gamma-treated human melanoma cells Colo 38

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.

Flow cytometry analysis (surface staining) of human peripheral blood cells using anti-HLA-ABCE (TP25.99SF) purified, GAM-APC.

Anti-HLA-ABCE Purified (clone TP25.99SF) works in WB application.

Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of JAR, JEG3, Raji, and K562 cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody TP25.99SF (2 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse IgG1 anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (1 µg/ml) was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.

HLA-ABCE molecules were detected at ~40 kDa in JAR and Raji cell lines.

Product specific references:

Tanabe M, Sekimata M, Ferrone S, Takiguchi M: Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain. J Immunol. 1992 May 15;148(10):3202-9.
PubMed

Desai SA, Wang X, Noronha EJ, Zhou Q, Rebmann V, Grosse-Wilde H, Moy FJ, Powers R, Ferrone S: Structural relatedness of distinct determinants recognized by monoclonal antibody TP25.99 on beta 2-microglobulin-associated and beta 2-microglobulin-free HLA class I heavy chains. J Immunol. 2000 Sep 15;165(6):3275-83.
PubMed

Perosa F, Luccarelli G, Prete M, Favoino E, Ferrone S, Dammacco F: Beta 2-microglobulin-free HLA class I heavy chain epitope mimicry by monoclonal antibody HC-10-specific peptide. J Immunol. 2003 Aug 15;171(4):1918-26.
PubMed

Moy FJ, Desai SA, Wang X, Noronha EJ, Zhou Q, Ferrone S, Powers R: Analysis by NMR spectroscopy of the structural homology between the linear and the cyclic peptide recognized by anti-human leukocyte antigen class I monoclonal antibody TP25.99. J Biol Chem. 2000 Aug 11;275(32):24679-85.
PubMed

Blaschitz A, Hutter H, Leitner V, Pilz S, Wintersteiger R, Dohr G, Sedlmayr P: Reaction patterns of monoclonal antibodies to HLA-G in human tissues and on cell lines: a comparative study. Hum Immunol. 2000 Nov;61(11):1074-85.
PubMed

Paul P, Rouas-Freiss N, Moreau P, Cabestre FA, Menier C, Khalil-Daher I, Pangault C, Onno M, Fauchet R, Martinez-Laso J, Morales P, Villena AA, Giacomini P, Natali PG, Frumento G, Ferrara GB, McMaster M, Fisher S, Schust D, Ferrone S, Dausset J, Geraghty D, Carosella ED: HLA-G, -E, -F preworkshop: tools and protocols for analysis of non-classical class I genes transcription and protein expression. Hum Immunol. 2000 Nov;61(11):1177-95.
PubMed