Rat IgG2b Isotype Control FITC

Rat IgG2b Isotype Control FITC
Regulatory status
RUO
Clone
RTG2B1-2
Format
FITC
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-169-C100
In stock
150.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD
Product details
Images
References
Isotype
Rat IgG2b
Specificity
This rat IgG2b kappa monoclonal antibody (clone RTG2B1-2, also known as G2B-1-2 or KLH/G2B-1-2) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Application
FC (QC tested)
Application details
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Concentration
1 mg/ml
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1F-169_FC_Profil Značení
Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using rat IgG2b Isotype control (RTG2B1-2) FITC antibody (concentration in sample 9 μg/ml).

Product specific references:

Chen L, Arora M, Yarlagadda M, Oriss TB, Krishnamoorthy N, Ray A, Ray P: Distinct responses of lung and spleen dendritic cells to the TLR9 agonist CpG oligodeoxynucleotide. J Immunol. 2006 Aug 15;177(4):2373-83.
PubMed
Mor A, Planer D, Luboshits G, Afek A, Metzger S, Chajek-Shaul T, Keren G, George J: Role of naturally occurring CD4+ CD25+ regulatory T cells in experimental atherosclerosis. Arterioscler Thromb Vasc Biol. 2007 Apr;27(4):893-900.
PubMed
Koduru S, Wong E, Strowig T, Sundaram R, Zhang L, Strout MP, Flavell RA, Schatz DG, Dhodapkar KM, Dhodapkar MV: Dendritic cell-mediated activation-induced cytidine deaminase (AID)-dependent induction of genomic instability in human myeloma. Blood. 2012 Mar 8;119(10):2302-9.
PubMed
Variant
0.1 mg
1F-169-C100
In stock
150.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD