Exbio — Research products — Antibodies — Isotype controls — Mouse IgG2b Isotype Control DyLight<sup>®</sup> 488

Mouse IgG2b Isotype Control DyLight® 488

Mouse IgG2b Isotype Control DyLight<sup>®</sup> 488
Regulatory status
RUO
Clone
MPC-11
Format
DyLight® 488
Application
WB, FC (QC tested), IHC(F), IHC(P), ICC
Excitation laser
blue (488 nm)
Variant
0.1 mg
L4-692-C100
In stock
190.00 USD
Variant
0.1 mg
L4-692-C100
In stock
190.00 USD
Contact distributor
Product details
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References
Isotype
Mouse IgG2b kappa
Specificity
This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
Application
WB, FC (QC tested), IHC(F), IHC(P), ICC
Application details
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Negative species
Mouse, Rat, Human
Immunogen
KLH-coupled trinitrophenol
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with DyLight® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
L4-692_FC_Profil Značení
Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG2b Isotype control (MPC-11) DyLight® 488 antibody (concentration in sample 9 μg/ml).

Product specific references:

Liang XS, Zhou Y, Li CZ, Wan MB: Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells. World J Gastroenterol. 2010 Feb 7;16(5):618-24.
PubMed
Dewals BG, Vanderplasschen A: Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues. Vet Res. 2011 Aug 22;42(1):95.
PubMed
Gupta D, Wang Q, Vinson C, Dziarski R: Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1. J Biol Chem. 1999 May 14;274(20):14012-20.
PubMed
Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15;111(10):5037-46.
PubMed
Im JS, Tapinos N, Chae GT, Illarionov PA, Besra GS, DeVries GH, Modlin RL, Sieling PA, Rambukkana A, Porcelli SA: Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells. J Immunol. 2006 Oct 15;177(8):5226-35.
PubMed
Variant
0.1 mg
L4-692-C100
In stock
190.00 USD
Variant
0.1 mg
L4-692-C100
In stock
190.00 USD
Contact distributor
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