Exbio — Research products — Antibodies — Isotype controls — Mouse IgG2a Isotype Control Purified Low Endotoxin

Mouse IgG2a Isotype Control Purified Low Endotoxin

Regulatory status

RUO

Clone

MOPC-173

Format

Purified Low Endotoxin

Application

ELISA, IP, WB, FC (QC tested), IHC(F), IHC(P), ICC, FUNC

Variant

0.1 mg

12-724-C100

In stock

115.50 USD

Variant

0.1 mg

12-724-C100

In stock

115.50 USD

Contact distributor

Product details

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Application

ELISA, IP, WB, FC (QC tested), IHC(F), IHC(P), ICC, FUNC

Application details

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

Negative species

Mouse, Rat, Human

Immunogen

The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Example of nonspecific mouse IgG2a (MOPC-173) purified antibody (low endotoxin) / GAM-APC signal (red) on human peripheral blood compared with blank (black).

Product specific references:

Fougereau M, Bourgois A, de Preval C, Rocca-Serra J, Schiff C: The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications. Ann Immunol (Paris). 1976 Sep-Oct;127(5):607-31.
PubMed

Baumal R, Scharff MD: Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone. J Immunol. 1976 Jan;116(1):65-74.
PubMed

Gupta V, Gylling A, Alonso JL, Sugimori T, Ianakiev P, Xiong JP, Arnaout MA: The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18. Blood. 2007 Apr 15;109(8):3513-20.
PubMed

Khoddami V, Cairns BR: Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP. Nat Protoc. 2014 Feb;9(2):337-61.
PubMed