Mouse IgG2a Isotype Control APC

Mouse IgG2a Isotype Control APC
Regulatory status
RUO
Clone
MOPC-173
Format
APC
Application
Excitation laser
red (633 nm)
Variant
0.1 mg
1A-724-C100
In stock
198.00 USD

0.025 mg
1A-724-C025
In stock
99.00 USD
Variant
0.1 mg
1A-724-C100
In stock
198.00 USD

0.025 mg
1A-724-C025
In stock
99.00 USD
Product details
Images
References
SDS download
Isotype
Mouse IgG2a kappa
Specificity
This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Application
Application details
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Negative species
Mouse, Rat, Human
Immunogen
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1A-724 FC
Flow cytometry analysis: Example of nonspecific mouse IgG2a (MOPC-173) APC signal on human peripheral blood; surface staining, 9 μg/ml.

Product specific references:

Fougereau M, Bourgois A, de Preval C, Rocca-Serra J, Schiff C: The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications. Ann Immunol (Paris). 1976 Sep-Oct;127(5):607-31.
PubMed
Baumal R, Scharff MD: Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone. J Immunol. 1976 Jan;116(1):65-74.
PubMed
Gupta V, Gylling A, Alonso JL, Sugimori T, Ianakiev P, Xiong JP, Arnaout MA: The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18. Blood. 2007 Apr 15;109(8):3513-20.
PubMed
Khoddami V, Cairns BR: Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP. Nat Protoc. 2014 Feb;9(2):337-61.
PubMed
Variant
0.1 mg
1A-724-C100
In stock
198.00 USD

0.025 mg
1A-724-C025
In stock
99.00 USD
Variant
0.1 mg
1A-724-C100
In stock
198.00 USD

0.025 mg
1A-724-C025
In stock
99.00 USD