Exbio — Research products — Antibodies — Isotype controls — Mouse IgG1 Isotype Control PerCP-Cy™5.5

Mouse IgG1 Isotype Control PerCP-Cy™5.5

Regulatory status

RUO

Clone

MOPC-21

Format

PerCP-Cy™5.5

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

T9-632-C100

In stock

275.00 USD

0.025 mg

T9-632-C025

Delivery 1 week

137.50 USD

Variant

0.1 mg

T9-632-C100

In stock

275.00 USD

0.025 mg

T9-632-C025

Delivery 1 week

137.50 USD

Contact distributor

Product details

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.

Application

FC (QC tested)

Application details

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

Negative species

Rat, Human

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy™5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG1 Isotype control (MOPC-21) PerCP-Cy™5.5 antibody (concentration in sample 15 μg/ml).

Product specific references:

Rebetz J, Tian D, Persson A, Widegren B, Salford LG, Englund E, Gisselsson D, Fan X: Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma. PLoS One. 2008 Apr 9;3(4):e1936.
PubMed

Bryceson YT, March ME, Barber DF, Ljunggren HG, Long EO: Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells. J Exp Med. 2005 Oct 3;202(7):1001-12.
PubMed

Wiendl H, Mitsdoerffer M, Schneider D, Melms A, Lochmuller H, Hohlfeld R, Weller M: Muscle fibres and cultured muscle cells express the B7.1/2-related inducible co-stimulatory molecule, ICOSL: implications for the pathogenesis of inflammatory myopathies. Brain. 2003 May;126(Pt 5):1026-35.
PubMed

Yates J, Rovis F, Mitchell P, Afzali B, Tsang JY, Garin M, Lechler RI, Lombardi G, Garden OA: The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol. 2007 Jun;19(6):785-99.
PubMed

Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15;111(10):5037-46.
PubMed

Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25.
PubMed