Clinical products
Premium antibodies (GMP)
Intracellular staining
Surface staining
Hematology
Kits
Lysing solutions
Premixed antibody combinations
Single color antibodies
Immunodeficiency
COVID-19 kits
Kits
Lysing solutions
Mitogens
Premixed antibody combinations
Single color antibodies
Reproductive immunology
Kits
Lysing solutions
Premixed antibody combinations
New products
Allergy
Kits
Recombinant allergens
Allergen extracts
Other allergens
Research products
Antibodies
CD and related antigens
Blood group antigens
Premixed antibody combinations
Serum, plasma, and secreted proteins
Cytoskeleton and ECM
Isotype controls
MHC antigens
Nucleus
Other intracellular antigens
Small organic molecules
Tags and labels
Microbiology and virology
Accessory reagents
Annexin binding
DNA-binding probes
Lineage cocktail
Lysing solutions
Recombinant proteins
Recombinant allergens
Animal
Food
House Dust Mite
Mold
Pollen
Venom
Kits
New products
Custom products
OEM and Commercial Supplies
About exbio
Company
Blog
Events and Conferences
Inquiries
Technical resources
International distributors
Contact
Jobs
E-mail
*
Password
*
Remember me
Forgotten password
Log in
Register
E-mail
Send request
Log in
Exbio
—
Research products
—
Antibodies
—
Cytoskeleton and ECM
—
Anti-Vimentin Purified
Anti-Vimentin Purified
Regulatory status
RUO
Antigen
Vimentin
Clone
VI-10
Format
Purified
Reactivity
Pig, Chicken, Rat, Human, Mouse
Application
IP, WB, IHC(P), ICC
Variant
0.1 mg
11-460-C100
In stock
160.00 USD
0.025 mg
11-460-C025
In stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD
0.1 mg
11-261-C100
In stock
80.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgM
Specificity
The antibody VI-10 reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types.
Application
IP, WB, IHC(P), ICC
Application details
Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml, positive tissue: skin fibroblast.
Reactivity
Pig, Chicken, Rat, Human, Mouse
Concentration
1 mg/ml
Preparation
Purified by precipitation and chromatography
Formulation
Tris buffered saline (TBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Immunocytochemistry staining of chicken postnatal erythrocytes. Tubulin (red) was stained with anti-alpha-tubulin (TU-01; cat. no. 11-250-C100), vimentin (green) with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue).
Immunohistochemistry staining of human skin (paraffin sections) using anti-vimentin (VI-10). Commercially tested by LifeSpan BioSciences.
Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions) Lnr = lysate (non-reducing conditions) IPr = immunoprecipitate (reducing conditions) IPnr = immunoprecipitate (non-reducing conditions)
General references:
Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Staskova Z: Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord. Histochem J. 1989 Dec;21(12):693-701.
PubMed
Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Dolezel S: Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord. Histochemistry. 1993 Dec;100(6):495-502.
PubMed
Product specific references:
Draberova E, Zikova M, Draber P: Monoclonal antibody VI-10 specific for vimentin. Folia Biol (Praha). 1999;45(1):35-6.
PubMed
Variant
0.1 mg
11-460-C100
In stock
160.00 USD
0.025 mg
11-460-C025
In stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD
0.1 mg
11-261-C100
In stock
80.00 USD
Contact distributor
Datasheet download
SDS download
CS
EN
Bulk requests
Technical support for this product
Continue to checkout
×
Zavřít