Anti-Vimentin Purified

Anti-Vimentin Purified
Regulatory status
RUO
Antigen
Vimentin
Clone
VI-10
Format
Purified
Reactivity
Pig, Chicken, Rat, Human, Mouse
Application
IP, WB, IHC(P), ICC
Variant
0.1 mg
11-460-C100
In stock

0.025 mg
11-460-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgM
Specificity
The antibody VI-10 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types.
Application
IP, WB, IHC(P), ICC
Reactivity
Pig, Chicken, Rat, Human, Mouse
Preparation
Purified by precipitation and chromatography
Formulation
Tris buffered saline (TBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-460 ICC
Immunocytochemistry staining of chicken postnatal erythrocytes. Tubulin (red) was stained with anti-alpha-tubulin (TU-01; cat. no. 11-250-C100), vimentin (green) with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue).
11-460 IP
Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions) Lnr = lysate (non-reducing conditions) IPr = immunoprecipitate (reducing conditions) IPnr = immunoprecipitate (non-reducing conditions)

General references:

Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Staskova Z: Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord. Histochem J. 1989 Dec;21(12):693-701.
PubMed
Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Dolezel S: Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord. Histochemistry. 1993 Dec;100(6):495-502.
PubMed

Product specific references:

Draberova E, Zikova M, Draber P: Monoclonal antibody VI-10 specific for vimentin. Folia Biol (Praha). 1999;45(1):35-6.
PubMed
Variant
0.1 mg
11-460-C100
In stock

0.025 mg
11-460-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock