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Exbio
—
Research products
—
Antibodies
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Cytoskeleton and ECM
—
Anti-Vimentin Purified
Anti-Vimentin Purified
Regulatory status
RUO
Antigen
Vimentin
Clone
VI-10
Format
Purified
Reactivity
Pig, Chicken, Rat, Human, Mouse
Application
IP, WB, IHC(P), ICC
Variant
0.1 mg
11-460-C100
In stock
160.00 USD
0.025 mg
11-460-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-460-C100
In stock
160.00 USD
0.025 mg
11-460-C025
Delivery 1 week
80.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgM
Specificity
The antibody VI-10 reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types.
Application
IP, WB, IHC(P), ICC
Application details
Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml, positive tissue: skin fibroblast.
Western blotting: Recommended dilution: 1-2 μg/ml.
Reactivity
Pig, Chicken, Rat, Human, Mouse
Concentration
1 mg/ml
Preparation
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Formulation
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Antigen description
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Entrez Gene ID
7431
UniProt ID
P08670
Immunocytochemistry staining of chicken postnatal erythrocytes. Tubulin (red) was stained with anti-alpha-tubulin (TU-01; cat. no. 11-250-C100), vimentin (green) with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue).
Immunohistochemistry staining of human skin (paraffin sections) using anti-vimentin (VI-10). Commercially tested by LifeSpan BioSciences.
Western blotting analysis of human vimentin using mouse monoclonal antibody VI-10 on lysates of MOLT-4 cell line (low expression), U87 cell line (positive) and Raji cell line (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-vimentin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for vimentin at approximately 55 kDa.
Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions) Lnr = lysate (non-reducing conditions) IPr = immunoprecipitate (reducing conditions) IPnr = immunoprecipitate (non-reducing conditions)
General references:
Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Staskova Z: Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord. Histochem J. 1989 Dec;21(12):693-701.
PubMed
Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Dolezel S: Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord. Histochemistry. 1993 Dec;100(6):495-502.
PubMed
Product specific references:
Draberova E, Zikova M, Draber P: Monoclonal antibody VI-10 specific for vimentin. Folia Biol (Praha). 1999;45(1):35-6.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
Variant
0.1 mg
11-460-C100
In stock
160.00 USD
0.025 mg
11-460-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-460-C100
In stock
160.00 USD
0.025 mg
11-460-C025
Delivery 1 week
80.00 USD
Contact distributor
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