Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Vimentin Purified

Anti-Vimentin Purified

Anti-Vimentin Purified
Regulatory status
RUO
Antigen
Vimentin
Clone
VI-01
Format
Purified
Reactivity
Mammalian
Application
WB, ICC
Variant
0.1 mg
11-254-C100
In stock
160.00 USD
0.025 mg
11-254-C025
Out of stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD
0.1 mg
11-261-C100
In stock
80.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgM
Specificity
The antibody VI-01 reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.
Application
WB, ICC
Application details
Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line.
Reactivity
Mammalian
Immunogen
Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
Concentration
1 mg/ml
Preparation
Purified by precipitation and chromatography
Formulation
Tris buffered saline (TBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-254 WB
Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4). Lane 1,2: immunostaining with anti-Vimentin (VI-01) Lane 3,4: immunostaining with anti-human Vimentin (VI-RE/1; cat. no. 11-369-C100)
11-254 WB_2
Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions) Lnr = lysate (non-reducing conditions) IPr = immunoprecipitate (reducing conditions) IPnr = immunoprecipitate (non-reducing conditions)

General references:

Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Staskova Z: Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord. Histochem J. 1989 Dec;21(12):693-701.
PubMed
Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Dolezel S: Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord. Histochemistry. 1993 Dec;100(6):495-502.
PubMed

Product specific references:

Bacáková L, Mares V, Bottone MG, Pellicciari C, Lisá V, Svorcík V: Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture. J Biomed Mater Res. 2000 Mar 5;49(3):369-79.
PubMed
Bacáková L, Mares V, Lysá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun;21(11):1173-9.
PubMed
Draberova E, Draber P, Havlicek F, Viklicky V: A common antigenic determinant of vimentin and desmin defined by monoclonal antibody. Folia Biol (Praha). 1986;32(5):295-303.
PubMed
Variant
0.1 mg
11-254-C100
In stock
160.00 USD
0.025 mg
11-254-C025
Out of stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD
0.1 mg
11-261-C100
In stock
80.00 USD
Contact distributor
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