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Exbio
—
Research products
—
Antibodies
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Cytoskeleton and ECM
—
Anti-Vimentin PE
Anti-Vimentin PE
Regulatory status
RUO
Antigen
Vimentin
Clone
VI-RE/1
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-369-C100
In stock
242.00 USD
0.025 mg
1P-369-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1P-369-C100
In stock
242.00 USD
0.025 mg
1P-369-C025
Delivery 1 week
121.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament intracellular protein expressed on a wide variety of mesenchymal and mesodermal cell types.
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Reactivity
Human
Negative species
Mouse, Pig
Immunogen
Bacterially expressed full-length human vimentin
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Antigen description
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Entrez Gene ID
7431
UniProt ID
P08670
Separation of ESS-1 cells (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with ESS-1 cells stained using anti-Vimentin (VI-RE/1) PE antibody (concentration in sample 0.56 μg/ml).
Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with ESS-1 cells stained using anti-Vimentin (VI-RE/1) PE antibody (concentration in sample 0.56 μg/ml).
Flow cytometry analysis (intracellular staining) of Vimentin expression in LEP-19 human fibroblast cell line using anti-human Vimentin (VI-RE/1) PE. Overlay with Isotype mouse IgG1 control (PPV-06; cat. no. 1P-457-C100).
General references:
Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Staskova Z: Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord. Histochem J. 1989 Dec;21(12):693-701.
PubMed
Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Dolezel S: Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord. Histochemistry. 1993 Dec;100(6):495-502.
PubMed
Product specific references:
Chen YK, Chang WS, Wu IC, Li LH, Yang SF, Chen JY, Hsu MC, Chen SH, Wu DC, Lee JM, Huang CH, Goan YG, Chou SH, Huang CT, Wu MT: Molecular characterization of invasive subpopulations from an esophageal squamous cell carcinoma cell line. Anticancer Res. 2010 Mar;30(3):727-36.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
1P-369-C100
In stock
242.00 USD
0.025 mg
1P-369-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1P-369-C100
In stock
242.00 USD
0.025 mg
1P-369-C025
Delivery 1 week
121.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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Clone
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Application
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