Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-TPX2 Purified

Anti-TPX2 Purified

Regulatory status

RUO

Antigen

TPX2

Clone

TPX2-01

Format

Purified

Reactivity

Rat, Mouse, Human

Application

ELISA, IP, WB, IHC(P), ICC

Variant

0.1 mg

11-818-C100

In stock

187.00 USD

Variant

0.1 mg

11-818-C100

In stock

187.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated intracellular critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.

Application

ELISA, IP, WB, IHC(P), ICC

Reactivity

Rat, Mouse, Human

Immunogen

Human TPX2

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

DIL2, HCTP4, REPP86

Antigen description

TPX2 is a microtubule-associated protein, which is a critical regulator of mitosis. At the beginning of mitosis, TPX2 is released and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. After completion of mitosis the TPX2 protein disappears, but has also role in DNA damage response. Its overexpression has been demonstrated in many types of carcinomas. TPX2 belongs to the markers of worse tumor prognosis. On the other hand, down-regulation of TPX2 can inhibit cancer cell proliferation, migration and invasion.

Entrez Gene ID 22974

UniProt ID Q9ULW0

Immunocytochemistry of TPX2 using monoclonal antibody TPX2-01 in glioblastoma cell line T98G: a) interphase, b) mitosis. c) Immunohistochemistry of TPX2 in U87MG human glioblastoma cells (orthotopic xenograft in murine brain). Bar: 20 μm.

Immunoprecipitation of TPX2 from nuclear extract of HEK293 cells using monoclonal antibody TPX2-01. 1) nuclear extract, 2) antibody, 3) immunoprecipitate, 4) carrier

Western blotting analysis of TPX2 using monoclonal antibody TPX2-01 in A) human cell lines, B) murine (P19, NIH 3T3, Neuro-2a) and rat (RBL-2H3, L6) cell lines.

General references:

Tomii C, Inokuchi M, Takagi Y, Ishikawa T, Otsuki S, Uetake H, Kojima K, Kawano T: TPX2 expression is associated with poor survival in gastric cancer. World J Surg Oncol. 2017 Jan 9;15(1):14. doi: 10.1186/s12957-016-1095-y
PubMed

Zhang H, Zhang T, You Z, Zhang Y: Positive Surgical Margin, HPV Persistence, and Expression of Both TPX2 and PD-L1 Are Associated with Persistence/Recurrence of Cervical Intraepithelial Neoplasia after Cervical Conization. PLoS One. 2015 Dec 1;10(12):e0142868.
PubMed

Miwa T, Kokuryo T, Yokoyama Y, Yamaguchi J, Nagino M: Therapeutic potential of targeting protein for Xklp2 silencing for pancreatic cancer. Cancer Med. 2015 Jul;4(7):1091-100
PubMed