Anti-TPX2 Purified

Anti-TPX2 Purified
Regulatory status
RUO
Antigen
TPX2
Clone
TPX2-01
Format
Purified
Reactivity
Rat, Mouse, Human
Application
ELISA, IP, WB, IHC(P), ICC
Variant
0.1 mg
11-818-C100
In stock
Variant
0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.
Application
ELISA, IP, WB, IHC(P), ICC
Reactivity
Rat, Mouse, Human
Immunogen
Human TPX2
Other names
DIL2, HCTP4, REPP86
Preparation
Purified from cell culture supernatant by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-818 ICC-IHC
Immunocytochemistry of TPX2 using monoclonal antibody TPX2-01 in glioblastoma cell line T98G: a) interphase, b) mitosis. c) Immunohistochemistry of TPX2 in U87MG human glioblastoma cells (orthotopic xenograft in murine brain). Bar: 20 μm.
11-818 IP
Immunoprecipitation of TPX2 from nuclear extract of HEK293 cells using monoclonal antibody TPX2-01. 1) nuclear extract, 2) antibody, 3) immunoprecipitate, 4) carrier
11-818 WB
Western blotting analysis of TPX2 using monoclonal antibody TPX2-01 in A) human cell lines, B) murine (P19, NIH 3T3, Neuro-2a) and rat (RBL-2H3, L6) cell lines.

General references:

Tomii C, Inokuchi M, Takagi Y, Ishikawa T, Otsuki S, Uetake H, Kojima K, Kawano T: TPX2 expression is associated with poor survival in gastric cancer. World J Surg Oncol. 2017 Jan 9;15(1):14. doi: 10.1186/s12957-016-1095-y
PubMed
Zhang H, Zhang T, You Z, Zhang Y: Positive Surgical Margin, HPV Persistence, and Expression of Both TPX2 and PD-L1 Are Associated with Persistence/Recurrence of Cervical Intraepithelial Neoplasia after Cervical Conization. PLoS One. 2015 Dec 1;10(12):e0142868.
PubMed
Miwa T, Kokuryo T, Yokoyama Y, Yamaguchi J, Nagino M: Therapeutic potential of targeting protein for Xklp2 silencing for pancreatic cancer. Cancer Med. 2015 Jul;4(7):1091-100
PubMed
Variant
0.1 mg
11-818-C100
In stock
Variant
0.1 mg
11-261-C100
In stock