Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-RLTPR Purified

Anti-RLTPR Purified

Regulatory status

RUO

Antigen

RLTPR

Clone

EM-53

Format

Purified

Reactivity

Mouse, Human

Application

WB, FC (QC tested)

Variant

0.1 mg

11-718-C100

In stock

180.00 USD

0.025 mg

11-718-C025

Out of stock

90.00 USD

Variant

0.1 mg

11-261-C100

In stock

180.00 USD

0.1 mg

11-261-C100

In stock

90.00 USD

Contact distributor

Product details

Images

References

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation.

Application

WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml, Intracellular staining.

Reactivity

Mouse, Human

Immunogen

Murine RLTPR

Other names

CARMIL2, LRRC16C

Concentration

1 mg/ml

Preparation

Purified from cell culture supernatant by protein-A affinity chromatography

Formulation

Phosphate buffered saline (PBS) solution with 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Separation of RLTPR transfected cells stained using anti-human RLTPR (EM-53) purified antibody (GAM FITC, concentration in sample 9 μg/ml, red-filled) from RLTPR transfected cells stained using mouse IgG1 isotype control (MOPC-21) purified antibody (GAM FITC, concentration in sample 9 μg/ml, black-dashed) in flow cytometry analysis (surface staining).

General references:

Schober T, Magg T, Laschinger M, Rohlfs M, Linhares ND, Puchalka J, Weisser T, Fehlner K, Mautner J, Walz C, Hussein K, Jaeger G, Kammer B, Schmid I, Bahia M, Pena SD, Behrends U, Belohradsky BH, Klein C, Hauck F: A human immunodeficiency syndrome caused by mutations in CARMIL2. Nat Commun. 2017 Jan 23;8:14209.
PubMed

Product specific references:

Wang Y, Ma CS, Ling Y, Bousfiha A, Camcioglu Y, Jacquot S, Payne K, Crestani E, Roncagalli R, Belkadi A, Kerner G, Lorenzo L, Deswarte C, Chrabieh M, Patin E, Vincent QB, Müller-Fleckenstein I, Fleckenstein B, Ailal F, Quintana-Murci L, Fraitag S, Alyanakian MA, Leruez-Ville M, Picard C, Puel A, Bustamante J, Boisson-Dupuis S, Malissen M, Malissen B, Abel L, Hovnanian A, Notarangelo L, Jouanguy E, Tangye SG, Béziat V, Casanova JL: Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations. J Exp Med. 2016 Oct 17;213(11):2413-2435.
PubMed

Roncagalli R, Cucchetti M, Jarmuzynski N, Grégoire C, Bergot E, Audebert S, Baudelet E, Menoita MG, Joachim A, Durand S, Suchánek M, Fiore F, Zhang L, Liang Y, Camoin L, Malissen M4, Malissen B: The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells. J Exp Med. 2016 Oct 17;213(11):2437-2457.
PubMed

Liang Y, Cucchetti M, Roncagalli R, Yokosuka T, Malzac A, Bertosio E, Imbert J, Nijman IJ, Suchanek M, Saito T, Wülfing C, Malissen B, Malissen M: The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells. Nat Immunol. 2013 Aug;14(8):858-66.
PubMed

Variant

0.1 mg

11-718-C100

In stock

180.00 USD

0.025 mg

11-718-C025

Out of stock

90.00 USD

Variant

0.1 mg

11-261-C100