Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-NHERF1 Purified

Anti-NHERF1 Purified

Regulatory status

RUO

Antigen

NHERF1

Clone

EBP-10

Format

Purified

Reactivity

Human

Application

IP, WB, IHC(P)

Variant

0.1 mg

11-348-C100

In stock

198.00 USD

0.025 mg

11-348-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-348-C100

In stock

198.00 USD

0.025 mg

11-348-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody EBP-10 reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.

Application

IP, WB, IHC(P)

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml.
Western blotting: Recommended dilution: 2 μg/ml, reducing conditions, positive control: MCF-7 cell line

Reactivity

Human

Immunogen

Bacterially produced recombinant full-length human NHERF1.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

EBP50, NPHLOP2, SLC9A3R1, SR1

Antigen description

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

Entrez Gene ID 9368

UniProt ID O14745

Western blotting analysis of human NHERF1 using mouse monoclonal antibody EBP-10 on lysates of Jurkat, MCF-7, and Daudi cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-NHERF1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. NHERF1 was detected around 50 kDa.

General references:

Lederer ED, Khundmiri SJ, Weinman EJ: Role of NHERF-1 in regulation of the activity of Na-K ATPase and sodium-phosphate co-transport in epithelial cells. J Am Soc Nephrol. 2003 Jul;14(7):1711-9.
PubMed

Brdickova N, Brdicka T, Andera L, Spicka J, Angelisova P, Milgram SL, Horejsi V: Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton. FEBS Lett. 2001 Oct 26;507(2):133-6.
PubMed

Weinman EJ, Cunningham R, Wade JB, Shenolikar S: The role of NHERF-1 in the regulation of renal proximal tubule sodium-hydrogen exchanger 3 and sodium-dependent phosphate cotransporter 2a. J Physiol. 2005 Aug 15;567(Pt 1):27-32.
PubMed

Wheeler D, Sneddon WB, Wang B, Friedman PA, Romero G: NHERF-1 and the cytoskeleton regulate the traffic and membrane dynamics of G protein-coupled receptors. J Biol Chem. 2007 Aug 24;282(34):25076-87.
PubMed

Morales FC, Takahashi Y, Momin S, Adams H, Chen X, Georgescu MM: NHERF1/EBP50 head-to-tail intramolecular interaction masks association with PDZ domain ligands. Mol Cell Biol. 2007 Apr;27(7):2527-37.
PubMed

Kreimann EL, Morales FC, de Orbeta-Cruz J, Takahashi Y, Adams H, Liu TJ, McCrea PD, Georgescu MM: Cortical stabilization of beta-catenin contributes to HERF1/EBP50 tumor suppressor function. Oncogene. 2007 Aug 9;26(36):5290-9.
PubMed

Product specific references:

He P, Zhao L, No YR, Karvar S, Yun CC: The NHERF1 PDZ1 domain and IRBIT interact and mediate the activation of Na+/H+ exchanger 3 by ANG II. Am J Physiol Renal Physiol. 2016 Aug 1;311(2):F343-51.
PubMed