Anti-Neurofilament M Purified

Anti-Neurofilament M Purified
Regulatory status
RUO
Antigen
Neurofilament M
Clone
NF-09
Format
Purified
Reactivity
Mammalian
Application
WB, IHC(P), ICC
Variant
0.1 mg
11-294-C100
In stock

0.025 mg
11-294-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG2a
Specificity
The antibody NF-09 reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species.
Application
WB, IHC(P), ICC
Reactivity
Mammalian
Immunogen
Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
Other names
NFM, NEFM, NEF3
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-294 WB
Western blotting analysis of neurofilament medium protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-09.
11-294 ICC
Immunocytochemistry staining of neurofilament medium protein in murine Neuro2A cells by antibody NF-09 conjugated with Dyomics 547 (red). DNA stained by Hoechst (blue).

General references:

Ohara O, Gahara Y, Miyake T, Teraoka H, Kitamura T: Neurofilament deficiency in quail caused by nonsense mutation in neurofilament-L gene. J Cell Biol. 1993 Apr;121(2):387-95.
PubMed
Rao MV, Campbell J, Yuan A, Kumar A, Gotow T, Uchiyama Y, Nixon RA: The neurofilament middle molecular mass subunit carboxyl-terminal tail domains is essential for the radial growth and cytoskeletal architecture of axons but not for regulating neurofilament transport rate. J Cell Biol. 2003 Dec 8;163(5):1021-31.
PubMed
Deng Y, Li B, Liu F, Iqbal K, Grundke-Iqbal I, Brandt R, Gong CX: Regulation between O-GlcNAcylation and phosphorylation of neurofilament-M and their dysregulation in Alzheimer disease. FASEB J. 2008 Jan;22(1):138-45.
PubMed

Product specific references:

Dráberová E, Sulimenko V, Vinopal S, Sulimenko T, Sládková V, D'Agostino L, Sobol M, Hozák P, Křen L, Katsetos CD, Dráber P: Differential expression of human γ-tubulin isotypes during neuronal development and oxidative stress points to a γ-tubulin-2 prosurvival function. FASEB J. 2017 May;31(5):1828-1846.
PubMed
Draberova E, Sulimenko V, Kukharskyy V, Draber P: Monoclonal antibody NF-09 specific for neurofilament protein NF-M. Folia Biol (Praha). 1999;45(4):163-5.
PubMed
Variant
0.1 mg
11-294-C100
In stock

0.025 mg
11-294-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock