Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Neurofilament H Purified

Anti-Neurofilament H Purified

Regulatory status

RUO

Antigen

Neurofilament H

Clone

NF-01

Format

Purified

Reactivity

Mammalian

Application

WB, IHC(P), ICC

Variant

0.1 mg

11-256-C100

In stock

176.00 USD

0.025 mg

11-256-C025

Delivery 1 week

88.00 USD

Variant

0.1 mg

11-256-C100

In stock

176.00 USD

0.025 mg

11-256-C025

Delivery 1 week

88.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody NF-01 recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa intracellular antigen) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Application

WB, IHC(P), ICC

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Mammalian

Immunogen

Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

NFH, NEFH, Neurofilament heavy chain, CMT2CC

Antigen description

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights – neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.

Entrez Gene ID 4744

UniProt ID P12036

Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with anti-neurofilament heavy protein (NF-01).

Western blotting analysis of human neurofilament H protein using mouse monoclonal antibody NF-01 on lysates of HEK-293 cell line, human brain lysate, and MCF-7 cell line (neurofilament non-expressing cell line; negative control) under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-neurofilament H monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Neurofilament H was detected around 180 kDa.

General references:

Cote F, Collard JF, Julien JP: Progressive neuronopathy in transgenic mice expressing the human neurofilament heavy gene: a mouse model of amyotrophic lateral sclerosis. Cell. 1993 Apr 9;73(1):35-46.
PubMed

Ohara O, Gahara Y, Miyake T, Teraoka H, Kitamura T: Neurofilament deficiency in quail caused by nonsense mutation in neurofilament-L gene. J Cell Biol. 1993 Apr;121(2):387-95.
PubMed

Brown RH: Amyotrophic lateral sclerosis: recent insights from genetics and transgenic mice. Cell. 1995 Mar 10;80(5):687-92.

Wild EJ, Petzold A, Keir G, Tabrizi SJ: Plasma neurofilament heavy chain levels in Huntington's disease. Neurosci Lett. 2007 May 7;417(3):231-3.
PubMed

Miyazawa I, Nakashima I, Petzold A, Fujihara K, Sato S, Itoyama Y: High CSF neurofilament heavy chain levels in neuromyelitis optica. Neurology. 2007 Mar 13;68(11):865-7.
PubMed

Petzold A, Keir G, Warren J, Fox N, Rossor MN: A systematic review and meta-analysis of CSF neurofilament protein levels as biomarkers in dementia. Neurodegener Dis. 2007;4(2-3):185-94.
PubMed

Product specific references:

Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Dolezel S: Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord. Histochemistry. 1993 Dec; 100(6):495-502.
PubMed