Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Neurofilament H Purified

Anti-Neurofilament H Purified

Anti-Neurofilament H Purified
Regulatory status
RUO
Antigen
Neurofilament H
Clone
NF-01
Format
Purified
Reactivity
Mammalian
Application
WB, IHC(P), ICC
Variant
0.1 mg
11-256-C100
In stock
160.00 USD
0.025 mg
11-256-C025
Out of stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD
0.1 mg
11-261-C100
In stock
80.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody NF-01 recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa intracellular antigen) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Application
WB, IHC(P), ICC
Reactivity
Mammalian
Immunogen
Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
Other names
NFH, NEFH, Neurofilament heavy chain, CMT2CC
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-256_IHC
Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with anti-neurofilament heavy protein (NF-01).

General references:

Cote F, Collard JF, Julien JP: Progressive neuronopathy in transgenic mice expressing the human neurofilament heavy gene: a mouse model of amyotrophic lateral sclerosis. Cell. 1993 Apr 9;73(1):35-46.
PubMed
Ohara O, Gahara Y, Miyake T, Teraoka H, Kitamura T: Neurofilament deficiency in quail caused by nonsense mutation in neurofilament-L gene. J Cell Biol. 1993 Apr;121(2):387-95.
PubMed
Brown RH: Amyotrophic lateral sclerosis: recent insights from genetics and transgenic mice. Cell. 1995 Mar 10;80(5):687-92.
Wild EJ, Petzold A, Keir G, Tabrizi SJ: Plasma neurofilament heavy chain levels in Huntington's disease. Neurosci Lett. 2007 May 7;417(3):231-3.
PubMed
Miyazawa I, Nakashima I, Petzold A, Fujihara K, Sato S, Itoyama Y: High CSF neurofilament heavy chain levels in neuromyelitis optica. Neurology. 2007 Mar 13;68(11):865-7.
PubMed
Petzold A, Keir G, Warren J, Fox N, Rossor MN: A systematic review and meta-analysis of CSF neurofilament protein levels as biomarkers in dementia. Neurodegener Dis. 2007;4(2-3):185-94.
PubMed

Product specific references:

Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Dolezel S: Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord. Histochemistry. 1993 Dec; 100(6):495-502.
PubMed
Variant
0.1 mg
11-256-C100
In stock
160.00 USD
0.025 mg
11-256-C025
Out of stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD
0.1 mg
11-261-C100
In stock
80.00 USD
Contact distributor
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