Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-N-sulfated heparan sulfate Biotin

Anti-N-sulfated heparan sulfate Biotin

Regulatory status

RUO

Antigen

N-sulfated heparan sulfate

Clone

HepSS-1

Format

Biotin

Reactivity

Species independent

Application

IP, IHC, WB, FC (QC tested), ICC

Variant

0.1 mg

1B-821-C100

In stock

198.00 USD

Variant

0.1 mg

1B-821-C100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The mouse monoclonal antibody HepSS-1 (also known as HepSS1) recognizes N-sulfated heparan sulfate (extracellular antigen) present in many species.

Application

IP, IHC, WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 3-12 μg/ml.

Reactivity

Species independent

Immunogen

MethA murine fibrosarcoma

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

N-sulfo-rich heparan sulfate

Antigen description

Heparan sulfate (HS) proteoglycans are expressed on cell surfaces and in the extracellular matrix, and take part in developmental, regenerative, as well as pathological processes. By interaction with extracellular matrix components, growth factors, enzymes, and their inhibitors, they regulate and influence tissue distribution of the cells and biological activities of the proteins. N-sulfated heparan sulfate proteoglycans are located in specific microdomains in the plasma membrane, independent on those formed around N-acetyl-rich heparan sulfate, and play different role in the cell signaling.

Separation of HUVEC cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) stained using Anti-N-sulfated heparan sulfate (HepSS-1) biotin antibody (concentration in sample 12 μg/ml, Streptavidin APC).

Product specific references:

Kure S, Yoshie O: A syngeneic monoclonal antibody to murine Meth-A sarcoma (HepSS-1) recognizes heparan sulfate glycosaminoglycan (HS-GAG): cell density and transformation dependent alteration in cell surface HS-GAG defined by HepSS-1. J Immunol. 1986 Dec 15;137(12):3900-8.
PubMed

van den Born J, Salmivirta K, Henttinen T, Ostman N, Ishimaru T, Miyaura S, Yoshida K, Salmivirta M: Novel heparan sulfate structures revealed by monoclonal antibodies. J Biol Chem. 2005 May 27;280(21):20516-23.
PubMed

Lipscombe RJ, Nakhoul AM, Sanderson CJ, Coombe DR: Interleukin-5 binds to heparin/heparan sulfate. A model for an interaction with extracellular matrix. J Leukoc Biol. 1998 Mar;63(3):342-50.
PubMed

Crim RL, Audet SA, Feldman SA, Mostowski HS, Beeler JA: Identification of linear heparin-binding peptides derived from human respiratory syncytial virus fusion glycoprotein that inhibit infectivity. J Virol. 2007 Jan;81(1):261-71. Epub 2006 Oct 18.
PubMed

Chung JS, Shiue LH, Duvic M, Pandya A, Cruz PD Jr, Ariizumi K: Sézary syndrome cells overexpress syndecan-4 bearing distinct heparan sulfate moieties that suppress T-cell activation by binding DC-HIL and trapping TGF-beta on the cell surface. Blood. 2011 Mar 24;117(12):3382-90.
PubMed

Kure S, Yoshie O, Aso H: Metastatic potential of murine B16 melanoma correlates with reduced surface heparan sulfate glycosaminoglycan. Jpn J Cancer Res. 1987 Nov;78(11):1238-45.
PubMed

Mii Y, Yamamoto T, Takada R, Mizumoto S, Matsuyama M, Yamada S, Takada S, Taira M: Roles of two types of heparan sulfate clusters in Wnt distribution and signaling in Xenopus. Nat Commun. 2017 Dec 7;8(1):1973.
PubMed