Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-MRCK alpha Purified

Anti-MRCK alpha Purified

Anti-MRCK alpha Purified
Regulatory status
RUO
Antigen
MRCK alpha
Clone
PAb (636)
Format
Purified
Reactivity
Human
Application
WB, ICC
Variant
0.1 mg
11-636-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Contact distributor
Product details
Images
References
Isotype
Rabbit polyclonal
Specificity
The polyclonal antibody anti-MRCK alpha recognizes myotonic dystrophy kinase-related Cdc42-binding kinase alpha (intracellular), a 180 kDa Ser/Thr kinase, which is responsible for reorganization of actin cytoskeleton.
Application
WB, ICC
Application details
Western blotting: Recommended dilution: 1:200.
Immunocytochemistry: PFA fixation can be used.
Reactivity
Human
Immunogen
a peptide of RDFDGEDSDSPRHST sequence
Other names
MRCKA, PK428, CDC42BPA
Concentration
0.25 mg/ml
Preparation
Purified from rabbit serum by affinity chromatography
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-636_ICC
Immunocytochemistry detection of colocalization between endogenous MRCK (red) and transferrin-Dyomics 547 (green) after 10 min. incubation of the cells (HeLa) with transferrin-Dyomics 547. Immunostaining with polyclonal anti-MRCK alpha.
11-636_ICC_2
Immunocytochemistry detection of colocalization between endogenous MRCK (red) and transfected MRCK-EGFP (green) HeLa cells. Immunostaining with polyclonal anti-MRCK alpha.

General references:

Tan I, Seow KT, Lim L, Leung T: Intermolecular and intramolecular interactions regulate catalytic activity of myotonic dystrophy kinase-related Cdc42-binding kinase alpha. Mol Cell Biol. 2001 Apr;21(8):2767-78.
PubMed
Choi SH, Czifra G, Kedei N, Lewin NE, Lazar J, Pu Y, Marquez VE, Blumberg PM: Characterization of the interaction of phorbol esters with the C1 domain of MRCK (myotonic dystrophy kinase-related Cdc42 binding kinase) alpha/beta. J Biol Chem. 2008 Apr 18;283(16):10543-9.
PubMed

Product specific references:

Cmejla R, Ptackova P, Petrak J, Savvulidi F, Cerny J, Sebesta O, Vyoral D: Human MRCKalpha is regulated by cellular iron levels and interferes with transferrin iron uptake. Biochem Biophys Res Commun. 2010 Apr 30;395(2):163-7.
PubMed
Variant
0.1 mg
11-636-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Contact distributor
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