Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-MAP2 Purified

Anti-MAP2 Purified

Regulatory status

RUO

Antigen

MAP2

Clone

MT-08

Format

Purified

Reactivity

Pig, Human, Mouse

Application

ELISA, IP, WB, IHC(F), IHC(P), ICC

Variant

0.1 mg

11-473-C100

In stock

176.00 USD

0.025 mg

11-473-C025

Delivery 1 week

88.00 USD

Variant

0.1 mg

11-473-C100

In stock

176.00 USD

0.025 mg

11-473-C025

Delivery 1 week

88.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MT-08 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab), an intracellular antigen.

Application

ELISA, IP, WB, IHC(F), IHC(P), ICC

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: brain.
Immunohistochemistry (frozen sections): Positive tissue: murine brain.
Immunoprecipitation: Positive material: porcine brain.
Western blotting: Positive control: porcine brain.
ELISA: Positive control: porcine brain.
Immunocytochemistry: Positive control: human neuroblastoma SH-SY5Y.

Reactivity

Pig, Human, Mouse

Immunogen

Microtubule protein (bovine brain) enriched for kinesin

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Microtubule-associated protein 2, MAP-2

Antigen description

MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.

Entrez Gene ID 4133

UniProt ID P11137

Western blotting analysis (reducing conditions) of microtubules partially purified from porcine brain lysate. Lane 1: immunostaining with anti-MAP2ab (MT-01; cat. no. 11-253-C100) Lane 2: immunostaining with anti-MAP2ab (MT-07; cat. no. 11-472-C100) Lane 3: immunostaining with anti-MAP2ab (MT-08)