Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Kinesin Purified

Anti-Kinesin Purified

Regulatory status

RUO

Antigen

Kinesin

Clone

PAb (434)

Format

Purified

Reactivity

Mouse, Pig, Human

Application

WB, ICC

Variant

0.1 mg

11-434-C100

In stock

180.00 USD

0.025 mg

11-434-C025

Delivery 1 week

90.00 USD

Variant

0.1 mg

11-434-C100

In stock

180.00 USD

0.025 mg

11-434-C025

Delivery 1 week

90.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rabbit polyclonal

Specificity

The polyclonal antibody detects total level of endogenous kinesin protein (intracellular antigen).

Application

WB, ICC

Reactivity

Mouse, Pig, Human

Immunogen

stalk domain of human kinesin (aa 331-906) expressed in E. coli (FKHC3)

Concentration

1 mg/ml

Preparation

Purified by ligand affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

KIN

Antigen description

Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.

Western blotting detection of kinesin in reduced lysates of human HEK cell line (1) and porcine brain (2) by rabbit polyclonal anti-kinesin.

Product specific references:

Malcova-Janatova I, Richterova V, Draber P, Hasek J: Preparation of human recombinant kinesin heavy chain and epitope mapping of its structural domains. Folia Microbiol (Praha). 2004;49(6):665-70.
PubMed