Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Kinesin Heavy Chain Purified

Anti-Kinesin Heavy Chain Purified

Regulatory status

RUO

Antigen

Kinesin Heavy Chain

Clone

KN-03

Format

Purified

Reactivity

Pig, Rat, Mouse, Human

Application

ICC

Variant

0.1 mg

11-391-C100

In stock

198.00 USD

0.025 mg

11-391-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-391-C100

In stock

198.00 USD

0.025 mg

11-391-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The antibody KN-03 recognizes heavy chain of conventional kinesin, a protein associated with intracellular vesicles, and with lower affinity with denaturated molecule. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments The antibody KN-03 well recognizes kinesin bound to taxol-stabilized microtubules.

Application

ICC

Reactivity

Pig, Rat, Mouse, Human

Immunogen

Enriched fraction of porcine brain kinesin.

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

KHC, KINHC, KIF5

Antigen description

Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.

Immunofluorescence staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using anti-Kinesin (KN-03). Nuclei were stained with DAPI (blue).

Product specific references:

Macurek L, Draberova E, Richterova V, Bohm KJ, Draber P: Monoclonal antibodies KN-02 and KN-03 against the heavy chain of kinesin. Hybrid Hybridomics. 2002 Dec;21(6):457-62.
PubMed