Anti-Kinesin Heavy Chain Purified

Anti-Kinesin Heavy Chain Purified
Regulatory status
RUO
Antigen
Kinesin Heavy Chain
Clone
KN-02
Format
Purified
Reactivity
Pig, Human, Rat, Mouse
Application
Variant
0.1 mg
11-390-C100
In stock
180.00 USD

0.025 mg
11-390-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Mouse IgM
Specificity
The antibody KN-02 recognizes heavy chain of conventional kinesin, a protein associated with intracellular vesicles, and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.
Application
Reactivity
Pig, Human, Rat, Mouse
Immunogen
Enriched fraction of porcine brain kinesin.
Concentration
1 mg/ml
Preparation
Purified by precipitation and chromatography
Formulation
Tris buffered saline (TBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
KHC, KINHC, KIF5
Antigen description
Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
11-390_ICC
Immunocytochemistry staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using anti-Kinesin (KN-02). Nuclei were stained with DAPI (blue).

Product specific references:

Macurek L, Draberova E, Richterova V, Bohm KJ, Draber P: Monoclonal antibodies KN-02 and KN-03 against the heavy chain of kinesin. Hybrid Hybridomics. 2002 Dec;21(6):457-62.
PubMed
Malcová-Janatová I, Richterová V, Dráber P, Hasek J: Preparation of human recombinant kinesin heavy chain and epitope mapping of its structural domains. Folia Microbiol (Praha). 2004;49(6):665-70.
PubMed
Variant
0.1 mg
11-390-C100
In stock
180.00 USD

0.025 mg
11-390-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD