Anti-GFAP Purified

Anti-GFAP Purified
Regulatory status
RUO
Antigen
GFAP
Clone
GA-5
Format
Purified
Reactivity
Pig, Human, Rat
Variant
0.1 mg
11-583-C100
In stock
160.00 USD

0.025 mg
11-583-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD

0.1 mg
11-261-C100
In stock
80.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody GA-5 reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Application details
Immunocytochemistry: Recommended dilution: 1-4 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/ml.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein per 1 ml lysate.
Reactivity
Pig, Human, Rat
Immunogen
Porcine spinal cord
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
GFAP, ALXDRD
Antigen description
GFAP (glial fibrillary acidic protein) was discovered by Bignami et al. (1972) as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein probably also plays a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the insulating layer (myelin) that covers nerve cells. Additionally, GFAP protein may assist in maintaining the protective barrier that allows only certain substances to pass between blood vessels and the brain (blood-brain barrier).In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer’s disease, epilepsy and multiple sclerosis.Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells and for distinguishing of neoplasms of astrocytic origin from other neoplasms in the central nervous system. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene (Brenner et al., 2001). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.
Entrez Gene ID 2670
UniProt ID P14136
11-583_IHC
Immunohistochemistry staining of human brain cortex (paraffin sections) using anti-GFAP (GA-5). Commercially tested by LifeSpan BioSciences.

General references:

Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE, Messing A: Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet. 2001 Jan;27(1):117-20.
PubMed

Product specific references:

Rungger-Brändle E, Dosso AA, Leuenberger PM: Glial reactivity, an early feature of diabetic retinopathy. Invest Ophthalmol Vis Sci. 2000 Jun;41(7):1971-80.
PubMed
Guillemin GJ, Wang L, Brew BJ: Quinolinic acid selectively induces apoptosis of human astrocytes: potential role in AIDS dementia complex. J Neuroinflammation. 2005 Jul 26;2:16.
PubMed
Joardar A, Sen AK, Das S: Docosahexaenoic acid facilitates cell maturation and beta-adrenergic transmission in astrocytes. J Lipid Res. 2006 Mar;47(3):571-81.
PubMed
Triviño A, Ramírez JM, Ramírez AI, Salazar JJ, García-Sanchez J: Retinal perivascular astroglia: an immunoperoxidase study. Vision Res. 1992 Sep;32(9):1601-7.
PubMed
Perng MD, Cairns L, van den IJssel P, Prescott A, Hutcheson AM, Quinlan RA: Intermediate filament interactions can be altered by HSP27 and alphaB-crystallin. J Cell Sci. 1999 Jul;112 ( Pt 13):2099-112.
PubMed
Variant
0.1 mg
11-583-C100
In stock
160.00 USD

0.025 mg
11-583-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD

0.1 mg
11-261-C100
In stock
80.00 USD