Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-GFAP Purified

Anti-GFAP Purified

Anti-GFAP Purified
Regulatory status
RUO
Antigen
GFAP
Clone
GF-01
Format
Purified
Reactivity
Pig, Cat, Human
Application
IP, WB, IHC(P), ICC
Variant
0.1 mg
11-255-C100
In stock
160.00 USD
0.025 mg
11-255-C025
Out of stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD
0.1 mg
11-261-C100
In stock
80.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody GF-01 reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Application
IP, WB, IHC(P), ICC
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: human brain (cortex, cerebellum). The antibody GF-01 strongly stains astrocytes in human brain tissue sections but it is essentially negative on mouse and rat tissues.
Immunocytochemistry: Recommended dilution: 5-10 μg/ml.
Reactivity
Pig, Cat, Human
Negative species
Rat
Immunogen
Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
Other names
GFAP, ALXDRD
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-255_IHC
Immunohistochemistry staining of human cerebellum (paraffin-embedded sections) with anti-GFAP (clone GF-01).
11-255_IHC_2
Immunohistochemistry staining of human brain cortex (paraffin-embedded sections) with anti-GFAP (clone GF-01). Commercially tested by LifeSpan BioSciences.

General references:

Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE, Messing A: Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet. 2001 Jan;27(1):117-20.
PubMed

Product specific references:

Lukas Z, Draber P, Bucek J, Draberova E, Viklicky V, Staskova Z: Expression of vimentin and glial fibrillary acidic protein in human developing spinal cord. Histochem J. 1989 Dec;21(12):693-701.
PubMed
Variant
0.1 mg
11-255-C100
In stock
160.00 USD
0.025 mg
11-255-C025
Out of stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD
0.1 mg
11-261-C100
In stock
80.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product